Reith M E, Cattolico R A
Biochemistry. 1985 May 7;24(10):2550-6. doi: 10.1021/bi00331a023.
The chloroplasts of chlorophytic and chromophytic plants exhibit significant morphological and biochemical differences. Presently, it is impossible to compare the influence of ctDNA on the structure and function of organelles within these two phylogenetic groups for no data exist in the literature on the profile of protein products synthesized by a chromophytic plastid. In this paper, the chloroplast DNA coded proteins of the chromophytic plant Olisthodiscus luteus are investigated by labeling isolated chloroplasts in vitro. Isolated plastids of excellent morphological condition are pulse labeled with [35S]methionine. Approximately 100 proteins are detected by two-dimensional gel electrophoresis and fluorography. However, these isolated plastids have a number of unusual characteristics: (1) they are photosynthetically inactive; (2) in vitro protein synthesis is light independent; (3) all proteins synthesized in vitro are membrane associated.
绿藻植物和色藻植物的叶绿体在形态和生化方面存在显著差异。目前，由于文献中没有关于色藻质体合成的蛋白质产物概况的数据，因此无法比较叶绿体DNA对这两个系统发育类群中细胞器结构和功能的影响。在本文中，通过体外标记分离的叶绿体，研究了色藻植物黄斑油球藻叶绿体DNA编码的蛋白质。形态状况良好的分离质体用[35S]甲硫氨酸进行脉冲标记。通过二维凝胶电泳和荧光自显影检测到大约100种蛋白质。然而，这些分离的质体具有许多不寻常的特征：（1）它们没有光合活性；（2）体外蛋白质合成不依赖光照；（3）体外合成的所有蛋白质都与膜相关。
