Reith M E, Cattolico R A
Biochemistry. 1985 May 7;24(10):2556-61. doi: 10.1021/bi00331a024.
Information on the ctDNA protein coding profile of the Chlorophyta, Rhodophyta, and Chromophyta might provide clues to the evolutionary mechanism(s) by which plants diverged into these three phylogenetic groups. The purpose of this study was to examine the ctDNA protein coding profile of the chromophytic plant Olisthodiscus luteus. Whole cells were labeled in the presence of cycloheximide, an inhibitor of cytoplasmic protein synthesis. Control experiments demonstrate that the chloroplast proteins labeled in vivo by this technique form a distinct subset of the total proteins synthesized by the cell. Approximately 50 plastid proteins (35 soluble, 15 membrane) were detected after two-dimensional gel electrophoresis and fluorography. Three ctDNA-coded proteins, the large subunit of ribulosebisphosphate carboxylase, the apoprotein of the P700-chlorophyll a-protein complex, and the "photogene" were identified. These proteins are also coded by chlorophytic ctDNA. Unexpectedly, the ctDNA of Olisthodiscus was shown to code for the small subunit of ribulosebisphosphate carboxylase. The gene for this enzyme subunit is nuclear coded in all chlorophytic plants that have been analyzed.
关于绿藻门、红藻门和褐藻门叶绿体DNA（ctDNA）蛋白质编码图谱的信息，可能为植物分化成这三个系统发育类群的进化机制提供线索。本研究的目的是检测褐藻门植物黄管藻（Olisthodiscus luteus）的ctDNA蛋白质编码图谱。在细胞质蛋白合成抑制剂环己酰亚胺存在的情况下对整个细胞进行标记。对照实验表明，通过该技术在体内标记的叶绿体蛋白构成了细胞合成的总蛋白中的一个独特子集。二维凝胶电泳和荧光自显影后检测到约50种质体蛋白（35种可溶性蛋白、15种膜蛋白）。鉴定出三种由ctDNA编码的蛋白，即核酮糖二磷酸羧化酶大亚基、P700 - 叶绿素a - 蛋白复合体的脱辅基蛋白和“光基因”。这些蛋白也由绿藻门的ctDNA编码。出乎意料的是，黄管藻的ctDNA被证明可编码核酮糖二磷酸羧化酶的小亚基。在所有已分析的绿藻门植物中，该酶亚基的基因都是由核编码的。
