苜蓿组织培养中的再生：通过固态 P-31 NMR 研究体细胞胚胎发生过程中的细胞内 pH 值变化。
Regeneration in Alfalfa Tissue Culture: Characterization of Intracellular pH During Somatic Embryo Production by Solid-State P-31 NMR.
机构信息
Physical Sciences Center, Monsanto Company, St. Louis, Missouri 63167.
出版信息
Plant Physiol. 1985 Nov;79(3):584-9. doi: 10.1104/pp.79.3.584.
Abstract
The composition of phosphorus-containing compounds of intact lyophilized alfalfa tissue has been determined, in part, by solid-state (31)P nuclear magnetic resonance. The tissue (Medicago sativa L., cv Regen S; and some of its crosses) was grown in culture under both nonregenerating and regenerating conditions, the latter enhanced by the addition of specific amino acids. Analysis of the (31)P nuclear magnetic resonance spectra shows that regeneration is favored when metabolism occurs without the production of a low average intracellular pH.
摘要
通过固态（31）P 核磁共振，确定了完整冻干紫花苜蓿组织中含磷化合物的组成。这些组织（紫花苜蓿 L.，Regen S 品种；及其一些杂交品种）在非再生和再生条件下在培养中生长，后者通过添加特定氨基酸得到增强。（31）P 核磁共振谱分析表明，在代谢过程中没有产生低平均细胞内 pH 值时，有利于再生。
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