缺氧条件下玉米根尖细胞质pH调节机制及其在缺氧存活中的作用。
Mechanisms of cytoplasmic pH regulation in hypoxic maize root tips and its role in survival under hypoxia.
作者信息
Roberts J K, Callis J, Wemmer D, Walbot V, Jardetzky O
出版信息
Proc Natl Acad Sci U S A. 1984 Jun;81(11):3379-83. doi: 10.1073/pnas.81.11.3379.
Abstract
We show that a transient lactic fermentation provides the signal triggering ethanol production in hypoxic maize root tips. The signal is cytoplasmic pH. This interaction between lactic and ethanolic fermentation permits tight cytoplasmic pH regulation during hypoxia--cytoplasmic pH remaining near neutrality for several hours. Mutant roots unable to synthesize ethanol can neither regulate cytoplasmic pH nor maintain ATP levels during extended periods of hypoxia and, like vertebrate tissues, are less tolerant of hypoxia than normal maize. This indicates that cytoplasmic pH regulation is an important factor in survival under hypoxia.
摘要
我们发现，短暂的乳酸发酵会产生信号，触发缺氧状态下玉米根尖中的乙醇生成。该信号是细胞质pH值。乳酸发酵和乙醇发酵之间的这种相互作用使得在缺氧期间能够严格调节细胞质pH值——细胞质pH值在数小时内保持接近中性。无法合成乙醇的突变体根在长时间缺氧期间既不能调节细胞质pH值，也不能维持ATP水平，并且与脊椎动物组织一样，比正常玉米对缺氧的耐受性更低。这表明细胞质pH值调节是缺氧条件下生存的一个重要因素。
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本文引用的文献
1
The effects of extracellular pH and buffer concentration on the efflux of lactate from frog sartorius muscle.细胞外pH值和缓冲液浓度对青蛙缝匠肌乳酸流出的影响。
J Physiol. 1975 Aug;250(1):1-22. doi: 10.1113/jphysiol.1975.sp011040.
2
Effects of o(2) concentration on rice seedlings.氧气浓度对水稻幼苗的影响。
Plant Physiol. 1983 Jan;71(1):30-4. doi: 10.1104/pp.71.1.30.
3
Regulation of Cytoplasmic and Vacuolar pH in Maize Root Tips under Different Experimental Conditions.不同实验条件下玉米根尖细胞质和液泡pH的调节
Plant Physiol. 1982 Jun;69(6):1344-7. doi: 10.1104/pp.69.6.1344.
4
Metabolic Activity and Energy Charge of Excised Maize Root Tips under Anoxia: CONTROL BY SOLUBLE SUGARS.缺氧条件下离体玉米根尖的代谢活性与能荷：可溶性糖的调控作用
Plant Physiol. 1980 Dec;66(6):1053-7. doi: 10.1104/pp.66.6.1053.
5
Soluble Sugars, Respiration, and Energy Charge during Aging of Excised Maize Root Tips.离体玉米根尖衰老过程中的可溶性糖、呼吸作用与能荷
Plant Physiol. 1980 Sep;66(3):516-9. doi: 10.1104/pp.66.3.516.
6
Gluconeogenesis in the castor bean endosperm: I. Changes in glycolytic intermediates.蓖麻籽胚乳中的糖异生作用：I. 糖酵解中间产物的变化
Plant Physiol. 1971 Jan;47(1):48-52. doi: 10.1104/pp.47.1.48.
7
Respiratory metabolism in buckwheat seedlings.荞麦幼苗的呼吸代谢
Plant Physiol. 1967 Aug;42(8):1042-52. doi: 10.1104/pp.42.8.1042.
8
Intracellular oxidation-reduction states in vivo.体内细胞内的氧化还原状态。
Science. 1962 Aug 17;137(3529):499-508. doi: 10.1126/science.137.3529.499.
9
The anaerobic proteins of maize.玉米的厌氧蛋白
Cell. 1980 Jul;20(3):761-7. doi: 10.1016/0092-8674(80)90322-0.
10
Intracellular pH measurements by 31P nuclear magnetic resonance. Influence of factors other than pH on 31P chemical shifts.通过31P核磁共振测量细胞内pH值。除pH值以外的因素对31P化学位移的影响。
Biochemistry. 1981 Sep 15;20(19):5389-94. doi: 10.1021/bi00522a006.
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