Lopez-Ruiz A, Verbelen J P, Roldan J M, Diez J
Departamento de Bioquímica, Facultad de Veterinaria, Universidad de Córdoba, Córdoba 14071, Spain.
Plant Physiol. 1985 Dec;79(4):1006-10. doi: 10.1104/pp.79.4.1006.
Antibodies against nitrate reductase from Monoraphidium braunii have been used to determine the antigenic relationships of nitrate reductases from different green algae. Nitrate reductases from Chlamydomonas reinhardii, Chlorella fusca, Dunaliella salina, and Scenedesmus obliquus, were inhibited by, and cross-reacted with, antibodies raised against the enzyme from Monoraphidium braunii.These antibodies were also used to determine, by immunoelectron microscopy, the intracellular location of nitrate reductase in the aforementioned green algae. In all cases, the enzyme was specifically located in the pyrenoid.
来自布朗单针藻的硝酸还原酶抗体已被用于确定不同绿藻中硝酸还原酶的抗原关系。莱茵衣藻、小球藻、盐生杜氏藻和斜生栅藻的硝酸还原酶被针对布朗单针藻中该酶产生的抗体所抑制,并与之发生交叉反应。这些抗体还被用于通过免疫电子显微镜确定上述绿藻中硝酸还原酶的细胞内定位。在所有情况下,该酶都特异性地定位于蛋白核中。