Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de Córdoba, Córdoba 14071, Spain.
Plant Physiol. 1991 Jul;96(3):699-704. doi: 10.1104/pp.96.3.699.
The distribution of nitrite reductase (EC 1.7.7.1) in the green algae Chlamydomonas reinhardtii, Monoraphidium braunii, Chlorella fusca, and Scenedesmus obliquus was studied by immunoelectron microscopy. The labeling of ultrathin cryosections was performed with anti-nitrite reductase antibodies followed by gold-labeled goat anti-rabbit antibodies. In C. reinhardtii sections, gold label was mainly associated with the pyrenoid, tonoplast, and plasmalemma. Significant labeling was also detected in the thylakoid region. In all other organisms, label density was lower but distributed in the same locations, except that the plasmalemma of S. obliquus was not significantly labeled. From estimates of the relative volume of different cell regions, we found that approximately 80% of the total enzyme is located in the chloroplastic region (thylakoids plus pyrenoid) of C. reinhardtii, M. braunii, and C. fusca, and 97% in the case of S. obliquus.
通过免疫电子显微镜研究了亚硝酸还原酶（EC 1.7.7.1）在绿藻莱茵衣藻、单形藻、褐指藻和斜生栅藻中的分布。用抗亚硝酸还原酶抗体对超薄冷冻切片进行标记，然后用金标记的兔抗山羊抗体进行标记。在莱茵衣藻切片中，金标记主要与淀粉核、液泡膜和质膜相关。在类囊体区域也检测到了显著的标记。在所有其他生物体中，标记密度较低，但分布位置相同，只是斜生栅藻的质膜没有明显标记。根据不同细胞区域相对体积的估计，我们发现大约 80%的总酶位于莱茵衣藻、单形藻和褐指藻的叶绿体区域（类囊体加淀粉核），而在斜生栅藻中则为 97%。
