Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-01, Japan.
Plant Physiol. 1986 Jan;80(1):85-90. doi: 10.1104/pp.80.1.85.
Protein composition and Mn abundance were compared between the two photosystem II (PSII) particle preparations obtained before and after photoactivation of the latent O(2)-evolving system in intermittently flashed wheat leaves. The following results have been obtained: (a) nonphotoactivated PSII particles were devoid of two extrinsic proteins which corresponded to the 24 and 16 kilodalton proteins in spinach particles, although the particles contained all the intrinsic proteins and the 33 kilodalton extrinsic protein. (b) The two extrinsic proteins absent in nonphotoactivated PSII particles were present in nonphotoactivated thylakoids, but were easily removed by a hypotonic shock followed by brief sonication. Such removal of the proteins did not occur in photoactivated thylakoids. (c) Nonphotoactivated PSII particles contained 1.5 Mn/400 chlorophyll, while photoactivated particles contained 8 Mn/400 chlorophyll. (d) Nonphotoactivated thylakoids contained 6 Mn/400 chlorophyll, but most of them were removed from thylakoids by a hypotonic shock in the presence of ethylenediaminetetraacetate. Such removal of Mn did not occur in photoactivated thylakoids.
在间歇性闪光小麦叶片中，潜在的 O2 释放系统被光激活前后，比较了两种光系统 II（PSII）颗粒制剂的蛋白质组成和 Mn 丰度。得到以下结果：(a) 非光激活 PSII 颗粒缺乏与菠菜颗粒中 24 和 16 千道尔顿蛋白相对应的两种外显子蛋白，尽管颗粒含有所有内在蛋白和 33 千道尔顿的外显子蛋白。(b) 非光激活 PSII 颗粒中不存在的两种外显子蛋白存在于非光激活的类囊体中，但通过低渗冲击后短暂超声很容易去除。这种蛋白质的去除不会发生在光激活的类囊体中。(c) 非光激活 PSII 颗粒中含有 1.5 Mn/400 叶绿素，而光激活颗粒中含有 8 Mn/400 叶绿素。(d) 非光激活的类囊体中含有 6 Mn/400 叶绿素，但在乙二胺四乙酸存在的低渗冲击下，它们中的大部分都从类囊体中去除。这种 Mn 的去除不会发生在光激活的类囊体中。
