Cohn M A, Hughes J A
Department of Plant Pathology and Crop Physiology, Louisiana Agricultural Experiment Station, Louisiana State University, Baton Rouge, Louisiana 70803.
Plant Physiol. 1986 Feb;80(2):531-3. doi: 10.1104/pp.80.2.531.
The activity of NaN(3) (0.5 millimolar), hydroxylamine-HCl (10-18 millimolar), and potassium cyanide (1 millimolar) as dormancy-breaking agents of dehulled red rice (Oryza sativa) is pH-dependent such that medium pH values favoring formation of the uncharged chemical species resulted in the highest germination percentages. There was no promotive effect of pH itself in the range of 3 to 10. The minimum contact times for maximum response (>/=90% germination) to NaN(3), KCN, and NH(2)OH-HCl are 8 hours at pH 4, 24 hours at pH 8, and 72 hours at pH 6 or 7, respectively, for exposure commencing at the start of imbibition. Dehulled seeds, imbibed first in water, show only slightly reduced germination when subsequently transferred to solutions of dormancy-breaking chemicals.Intact seeds remain dormant in the presence of NaN(3), KCN, or NH(2)OH-HCl unless partially dry-afterripened. The pH dependence of these chemicals is reduced in intact, afterripening seeds.
NaN₃（0.5毫摩尔）、盐酸羟胺（10 - 18毫摩尔）和氰化钾（1毫摩尔）作为糙米（水稻）休眠破除剂的活性取决于pH值，即有利于形成不带电荷化学物质的中等pH值会导致最高的发芽率。在3至10的范围内，pH本身没有促进作用。对于NaN₃、KCN和NH₂OH - HCl，从吸胀开始时起暴露，达到最大响应（≥90%发芽）的最短接触时间分别为pH 4时8小时、pH 8时24小时、pH 6或7时72小时。先在水中吸胀的去壳种子，随后转移到休眠破除化学物质溶液中时，发芽率仅略有降低。完整种子在NaN₃、KCN或NH₂OH - HCl存在下保持休眠，除非经过部分干燥后熟。在完整的后熟种子中，这些化学物质对pH的依赖性降低。
