Southwick S M, Chung A, Davenport T L, Ryan J W
Tropical Research and Education Center, Homestead, Florida 33031.
Plant Physiol. 1986 May;81(1):323-5. doi: 10.1104/pp.81.1.323.
A simple and rapid technique was developed to synthesize abscisic acid glucose ester. The free acid of abscisic acid (ABA) was combined with CsHCO(3) to form the Cs salt of ABA. The Cs salt of ABA was then combined with acetobromo-alpha-d-glucose tetraacetate, and the tetraacetate derivative of abscisic acid glucose ester was formed. Acetate groups were selectively removed from the glucose moiety with a crude enzyme preparation derived from Helianthus annuus seeds. Abscisic acid glucose ester was purified via silica gel column chromatography and identified by micro NMR.
开发了一种简单快速的技术来合成脱落酸葡萄糖酯。脱落酸（ABA）的游离酸与碳酸氢铯（CsHCO₃）结合形成ABA的铯盐。然后将ABA的铯盐与乙酰溴-α-D-葡萄糖四乙酸酯结合，形成脱落酸葡萄糖酯的四乙酸酯衍生物。用源自向日葵种子的粗酶制剂从葡萄糖部分选择性地除去乙酰基。通过硅胶柱色谱法纯化脱落酸葡萄糖酯，并通过微核磁共振进行鉴定。
