Department of Vegetable Crops, University of California, Davis, California 95616.
Plant Physiol. 1986 Jul;81(3):929-30. doi: 10.1104/pp.81.3.929.
Macerase, a pectinase-containing enzyme mixture, was used to digest cell walls isolated from cultured pear cells. Following digestion, the reaction mixture was boiled to inactivate enzymes. Addition of soluble aliquots of the mixture to suspension cultures of pear cells led to a rapid and transient production of ethylene by the cells.
果胶酶混合物 Macerase 被用于消化从培养的梨细胞中分离出的细胞壁。消化后,将反应混合物煮沸以灭活酶。将混合物的可溶性部分添加到梨细胞的悬浮培养物中,导致细胞迅速产生短暂的乙烯。