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豆原细胞中乙烯和诱导子对几丁质酶基因启动子的调控。

Regulation of a chitinase gene promoter by ethylene and elicitors in bean protoplasts.

机构信息

Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102.

出版信息

Plant Physiol. 1991 Sep;97(1):433-9. doi: 10.1104/pp.97.1.433.

DOI:10.1104/pp.97.1.433
PMID:16668405
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1081017/
Abstract

Chitinase gene expression has been shown to be transcriptionally regulated by a number of inducers, including ethylene, elicitors, and pathogen attack. To investigate the mechanism(s) responsible for induction of chitinase gene expression in response to various stimuli, we have developed a transient gene expression system in bean (Phaseolus vulgaris) protoplasts that is responsive to ethylene and elicitor treatment. This system was used to study the expression of a chimeric gene composed of the 5' flanking sequences of a bean endochitinase gene fused to the reporter gene beta-glucuronidase linked to a 3' fragment from nopaline synthase. Addition of 1-aminocyclopropane-1-carboxylic acid, the direct precursor of ethylene, or elicitors such as chitin oligosaccharides or cell wall fragments derived from Colletotrichum lagenarium, to transformed protoplasts resulted in a rapid and marked increase in the expression of the chimeric gene. The kinetics and dose response for these treatments were similar to those observed for the native gene in vivo. Analyses of 5' deletion mutants in the protoplast system indicated that DNA sequences located between -305 and -236 are important for both ethylene and elicitor induction of the reporter gene.

摘要

几丁质酶基因的表达已被证明受到许多诱导物的转录调控，包括乙烯、激发子和病原体攻击。为了研究对各种刺激物产生几丁质酶基因表达诱导的机制，我们在菜豆（Phaseolus vulgaris）原生质体中开发了一种对乙烯和激发子处理有反应的瞬时基因表达系统。该系统用于研究由菜豆内切几丁质酶基因的 5'侧翼序列与报告基因β-葡聚糖酶融合，并与来自胭脂碱合酶的 3'片段连接而成的嵌合基因的表达。添加乙烯的直接前体 1-氨基环丙烷-1-羧酸或激发子，如几丁寡糖或来自胶孢炭疽菌的细胞壁片段，可导致转化的原生质体中嵌合基因的表达迅速而显著增加。这些处理的动力学和剂量反应与体内观察到的天然基因相似。在原生质体系统中对 5'缺失突变体的分析表明，位于-305 到-236 之间的 DNA 序列对于乙烯和激发子诱导报告基因都是重要的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e6c/1081017/945029265c61/plntphys00696-0445-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e6c/1081017/945029265c61/plntphys00696-0445-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e6c/1081017/945029265c61/plntphys00696-0445-a.jpg

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Plant Physiol. 1987 Aug;84(4):1286-90. doi: 10.1104/pp.84.4.1286.
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The induction of ethylene production from pear cell culture by cell wall fragments.梨细胞培养物细胞壁碎片诱导乙烯产生。
Nat Protoc. 2014 Apr;9(4):939-49. doi: 10.1038/nprot.2014.061. Epub 2014 Mar 27.
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