Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340.
Plant Physiol. 1986 Sep;82(1):333-5. doi: 10.1104/pp.82.1.333.
A phytase was isolated and partially purified from the pollen of Lilium longiflorum Thumb. Optimum activity was at pH 8.0. The phytase was activated by Ca(2+) and Sr(2+) but not by the other divalent cations tested. Activity was inhibited by ethylenediaminetetraacetate. The phytase had a temperature optimum of 55 to 60 degrees C and an activation energy of about 12,700 calories/mole. Extraction of L. longiflorum pollen with 0.1% Triton X-100 increased recovery of the phytase by nearly 4-fold. The phytase had a molecular weight of about 88,000 as determined by gel filtration chromatography and a K(m) value of 7.2 micromolar for phytic acid in the presence of Ca(2+).
从百合花粉中分离并部分纯化了一种植酸酶。最适活性在 pH8.0。该植酸酶被 Ca(2+) 和 Sr(2+) 激活，但被测试的其他二价阳离子不激活。活性被乙二胺四乙酸抑制。植酸酶的最适温度为 55 至 60°C，活化能约为 12700 卡路里/摩尔。用 0.1% Triton X-100 提取百合花粉可使植酸酶的回收率提高近 4 倍。凝胶过滤层析法测定该植酸酶的分子量约为 88000，在 Ca(2+)存在下，植酸的 K(m)值为 7.2 微摩尔。
