Boss W F, Massel M O
Biochem Biophys Res Commun. 1985 Nov 15;132(3):1018-23. doi: 10.1016/0006-291x(85)91908-4.
Polyphosphoinositides have been isolated from wild carrot cells grown in suspension culture. This is the first report of polyphosphoinositides in plant cells. The phospholipids were identified by comigration with known standards on thin-layer plates. After overnight labeling of the cells with myo-[2-3H] inositol, the phosphoinositides as percent recovered inositol were 93% phosphatidylinositol., 3.7% lysophosphatidylinositol, 1.7% phosphatidylinositol monophosphate, 0.8% phosphatidylinositol bisphosphate.
多磷酸肌醇已从悬浮培养的野生胡萝卜细胞中分离出来。这是植物细胞中多磷酸肌醇的首次报道。通过在薄板上与已知标准品共迁移来鉴定磷脂。在用肌醇-[2-³H]对细胞进行过夜标记后，以回收的肌醇百分比计，磷酸肌醇分别为：93%磷脂酰肌醇、3.7%溶血磷脂酰肌醇、1.7%磷脂酰肌醇单磷酸、0.8%磷脂酰肌醇二磷酸。
