Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, P. O. Box 19687, New Orleans, Louisiana 70179.
Plant Physiol. 1986 Nov;82(3):724-8. doi: 10.1104/pp.82.3.724.
Two photoperiodic cotton (Gossypium hirsutum L.) strains (T185 and T466) which had been empirically selected because of poor performance and two strains (T25 and T256) selected because of enhanced performance under field water stress were evaluated for stress-induced changes in their organic acids and carbohydrates. Profiles and quantitation of organic acids and carbohydrates from aqueous extractions of cotton leaf tissue were determined by high performance liquid chromatography. In all cases, the water-stressed plants showed two to five times greater amounts of organic acids and carbohydrates over the values determined for the irrigated samples. Under stress, sucrose accumulation was observed in wilting strains (poor performers) probably related to rate of translocation out of the leaf. The most dramatic response to water stress was the accumulation of citric acid in strains T25 and T256 as compared to T185 and T466. Citric/malic acid ratios for both the irrigated and water-stressed samples of T25 and T256 were twice those of T185 and T466.
两种光周期棉花（Gossypium hirsutum L.）品系（T185 和 T466）因表现不佳而被经验性选择，两种品系（T25 和 T256）因在田间水分胁迫下表现增强而被选择，它们的有机酸和碳水化合物在胁迫下发生的变化进行了评估。通过高效液相色谱法测定了从棉花叶片组织水提物中有机酸和碳水化合物的图谱和定量。在所有情况下，与灌溉样本相比，水分胁迫下的植物的有机酸和碳水化合物含量高出两到五倍。在胁迫下，萎蔫品系（表现不佳的品系）中观察到蔗糖积累，这可能与蔗糖从叶片中的转运速度有关。与 T185 和 T466 相比，T25 和 T256 对水分胁迫的最显著响应是柠檬酸的积累。T25 和 T256 的灌溉和水分胁迫样本的柠檬酸/苹果酸比值是 T185 和 T466 的两倍。
