C3 植物中磷酸烯醇丙酮酸羧化酶的固碳作用
Anapleurotic CO(2) Fixation by Phosphoenolpyruvate Carboxylase in C(3) Plants.
机构信息
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706.
出版信息
Plant Physiol. 1987 May;84(1):58-60. doi: 10.1104/pp.84.1.58.
Abstract
The role of phosphoenolpyruvate carboxylase in photosynthesis in the C(3) plant Nicotiana tabacum has been probed by measurement of the (13)C content of various materials. Whole leaf and purified ribulose bisphosphate carboxylase are within the range expected for C(3) plants. Aspartic acid purified following acid hydrolysis of this ribulose bisphosphate carboxylase is enriched in (13)C compared to whole protein. Carbons 1-3 of this aspartic acid are in the normal C(3) range, but carbon-4 (obtained by treatment of the aspartic acid with aspartate beta-decarboxylase) has an isotopic composition in the range expected for products of C(4) photosynthesis (-5 per thousand), and it appears that more than half of the aspartic acid is synthesized by phosphoenolpyruvate carboxylase using atmospheric CO(2)/HCO(3) (-). Thus, a primary role of phosphoenolpyruvate carboxylase in C(3) plants appears to be the anapleurotic synthesis of four-carbon acids.
摘要
通过测量各种物质的 (13)C 含量，研究了磷酸烯醇丙酮酸羧化酶在 C(3) 植物烟草中的光合作用中的作用。整叶和纯化的核酮糖二磷酸羧化酶处于 C(3) 植物的预期范围内。与全蛋白相比，用酸水解这种核酮糖二磷酸羧化酶得到的天冬氨酸在 (13)C 中富集。这种天冬氨酸的碳 1-3 在正常的 C(3) 范围内，但碳-4（通过用天冬氨酸-β-脱羧酶处理天冬氨酸获得）的同位素组成处于 C(4) 光合作用产物的预期范围内（-5 千分之），并且似乎超过一半的天冬氨酸是由磷酸烯醇丙酮酸羧化酶利用大气 CO(2)/HCO(3)（-）合成的。因此，磷酸烯醇丙酮酸羧化酶在 C(3) 植物中的主要作用似乎是四碳酸的回补合成。
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