丁硫氨酸亚砜胺对经镉、锌或铜处理的悬浮培养烟草细胞中镉结合肽水平的影响。
Effects of buthionine sulfoximine on cd-binding Peptide levels in suspension-cultured tobacco cells treated with cd, zn, or cu.
作者信息
Reese R N, Wagner G J
机构信息
Department of Agronomy, ASCN, N212, University of Kentucky, Lexington, Kentucky 40546-0091.
出版信息
Plant Physiol. 1987 Jul;84(3):574-7. doi: 10.1104/pp.84.3.574.
Inhibition of cell growth and accumulation of Cd-binding peptide were measured in cultured tobacco cells exposed to buthionine sulfoximine. This inhibitor of glutathione metabolism caused little or no reduction of growth (at 0.1 millimolar) in the absence of Cd, but growth was greatly reduced in cultures exposed to buthionine sulfoximine and >/=22 micromolar Cd. Decreased cell growth was directly correlated with decreased levels of Cd-binding peptide and increased levels of what is thought to be free Cd. Zinc inhibited growth of tobacco cells only at the highest levels examined (900-1800 micromolar Zn), but buthionine sulfoximine had no additional significant effect. Similar results were observed for Cu (45-90 micromolar). Results suggest that synthesis of plant Cd-peptide involves gamma-glutamylcysteine synthetase or a related enzyme and that Zn accumulation in tobacco cells does not cause formation of significant Cd-peptide ligand.
在暴露于丁硫氨酸亚砜胺的培养烟草细胞中，检测了细胞生长抑制和镉结合肽的积累情况。这种谷胱甘肽代谢抑制剂在无镉的情况下对生长几乎没有或没有降低作用（在0.1毫摩尔时），但在暴露于丁硫氨酸亚砜胺和≥22微摩尔镉的培养物中，生长大幅降低。细胞生长的减少与镉结合肽水平的降低以及被认为是游离镉的水平升高直接相关。锌仅在检测的最高水平（900 - 1800微摩尔锌）时抑制烟草细胞生长，但丁硫氨酸亚砜胺没有额外的显著影响。对于铜（45 - 90微摩尔）也观察到了类似结果。结果表明，植物镉肽的合成涉及γ-谷氨酰半胱氨酸合成酶或一种相关酶，并且烟草细胞中锌的积累不会导致形成显著的镉肽配体。
Zotero 插件