特定番茄细胞系中植物螯合肽的积累与镉耐受性
Phytochelatin accumulation and cadmium tolerance in selected tomato cell lines.
作者信息
Gupta S C, Goldsbrough P B
机构信息
Department of Horticulture, Purdue University, West Lafayette, Indiana 47907.
出版信息
Plant Physiol. 1991 Sep;97(1):306-12. doi: 10.1104/pp.97.1.306.
Abstract
Four cell lines of tomato, Lycopersicon esculentum Mill. cv VFNT-Cherry, were selected for their ability to grow in the presence of up to 6 millimolar CdCl(2). The intracellular Cd concentration in these cells was at least 2.3 times higher than in the medium. Growth in media containing higher concentrations of Cd was accompanied by increased production of Cd-binding phytochelatins and a trend toward accumulation of higher molecular weight phytochelatins. At least 90% of the Cd in the most tolerant cells was associated with Cd-phytochelatin complexes. Cell lines maintained an increased tolerance of Cd in the absence of continuous selection pressure.
摘要
选择了四个番茄品种（Lycopersicon esculentum Mill. cv VFNT-Cherry）的细胞系，因其在高达6毫摩尔CdCl₂存在的情况下仍具有生长能力。这些细胞内的镉浓度比培养基中的至少高2.3倍。在含有更高浓度镉的培养基中生长伴随着镉结合植物螯合肽产量的增加以及高分子量植物螯合肽积累的趋势。在耐受性最强的细胞中，至少90%的镉与镉 - 植物螯合肽复合物相关。在没有持续选择压力的情况下，细胞系对镉的耐受性仍保持增强。
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本文引用的文献
1
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2
Poly(gamma-glutamylcysteinyl)glycine Synthesis in Datura innoxia and Binding with Cadmium : Role in Cadmium Tolerance.紫花曼陀罗中聚（γ-谷氨酰半胱氨酰）甘氨酸的合成及其与镉的结合：在镉耐受性中的作用
Plant Physiol. 1989 Feb;89(2):700-6. doi: 10.1104/pp.89.2.700.
3
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4
Amino Acid Metabolism of Lemna minor L. : II. Responses to Chlorsulfuron.浮萍（Lemna minor L.）的氨基酸代谢：II. 对氯磺隆的反应
Plant Physiol. 1987 Jul;84(3):775-80. doi: 10.1104/pp.84.3.775.
5
Effects of buthionine sulfoximine on cd-binding Peptide levels in suspension-cultured tobacco cells treated with cd, zn, or cu.丁硫氨酸亚砜胺对经镉、锌或铜处理的悬浮培养烟草细胞中镉结合肽水平的影响。
Plant Physiol. 1987 Jul;84(3):574-7. doi: 10.1104/pp.84.3.574.
6
Selection, Isolation, and Characterization of Cadmium-Resistant Datura innoxia Suspension Cultures.抗镉的紫花曼陀罗悬浮培养物的筛选、分离与特性研究
Plant Physiol. 1984 Aug;75(4):914-8. doi: 10.1104/pp.75.4.914.
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Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific gamma-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase).植物重金属结合肽——植物螯合肽，由特定的γ-谷氨酰半胱氨酸二肽转移酶（植物螯合肽合酶）从谷胱甘肽合成。
Proc Natl Acad Sci U S A. 1989 Sep;86(18):6838-42. doi: 10.1073/pnas.86.18.6838.
8
Phytochelatins, a class of heavy-metal-binding peptides from plants, are functionally analogous to metallothioneins.植物来源的植物螯合肽是一类重金属结合肽，在功能上与金属硫蛋白类似。
Proc Natl Acad Sci U S A. 1987 Jan;84(2):439-43. doi: 10.1073/pnas.84.2.439.
9
Tissue sulfhydryl groups.组织巯基
Arch Biochem Biophys. 1959 May;82(1):70-7. doi: 10.1016/0003-9861(59)90090-6.
10
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