Department of Horticulture, Oregon State University, Corvallis, Oregon 97331.
Plant Physiol. 1987 Dec;85(4):1089-93. doi: 10.1104/pp.85.4.1089.
The alt (albina-terminalis) mutant of Pisum sativum L. germinates normally, produces several nodes, and then above a sharp transition produces 2 to 3 bleached nodes, ceases growth, and eventually dies. Green nodes have normal chlorophyll content, absorption spectra, photosynthetic rates, and ultrastructure. In bleaching tissues, the chloroplasts degenerate rapidly, followed by extensive disruption and loss of the remaining cytoplasm and organelles. Application of tissue extracts of normal genotypes of pea, corn, and bean stimulates apical development of alt. The resulting tissues have essentially normal structure and function. Application of thiamine, thiamine monophosphate, and thiamine pyrophosphate also stimulate normal apical development at concentrations of 1 micromolar and above. Partial characterization of the stimulus from pea seed extracts is consistent with thiamine as the active factor.
豌豆的 albina-terminalis（白化终端）突变体正常发芽，产生几个节，然后在一个急剧转变处产生 2 到 3 个白化节，停止生长，最终死亡。绿色节具有正常的叶绿素含量、吸收光谱、光合速率和超微结构。在白化组织中，叶绿体迅速退化，随后剩余细胞质和细胞器广泛解体和丧失。豌豆、玉米和豆科植物正常基因型的组织提取物的应用刺激了 albina-terminalis 的顶端发育。所产生的组织具有基本正常的结构和功能。应用 1 微摩尔及以上浓度的硫胺素、硫胺素单磷酸盐和硫胺素焦磷酸盐也可刺激正常的顶端发育。豌豆种子提取物刺激物的部分特征与硫胺素作为活性因子一致。
