Carnegie Institution, Stanford, California 94305.
Plant Physiol. 1972 Mar;49(3):421-9. doi: 10.1104/pp.49.3.421.
We have matched the red absorption band measured at -196 C in a variety of chloroplast preparations with four major component curves representing forms of chlorophyll a having peaks at 661.6, 669.6, 677.1, and 683.7 nanometers. Chloroplast fractions enriched in one or the other of the two photochemical systems both contain these four major components, but system 1 preparations contain relatively more chlorophyll a 684. Chlorophyll a 677 and chlorophyll a 684 have greater bandwidths in system 1. Bands at longer wavelengths near 693 and 704 nanometers also often occur, but with far smaller heights than the above major bands. The longer wavelength bands are more common in system 1 than in system 2. In system 1 the half-widths of the four major bands in typical spectra average 11.3, 10.0, 10.3, and 10.8 nanometers while in system 2 they are 11.6, 9.8, 9.4, and 9.6 nanometers. Some spectra with sharper and some with wider bands were found, but the wavelengths were identical.
我们将在各种叶绿体制剂中测量到的-196°C 处的红色吸收带与代表叶绿素 a 形式的四个主要组分曲线相匹配，这些形式的叶绿素 a 在 661.6、669.6、677.1 和 683.7 纳米处具有峰值。富含两个光化学系统之一或另一个的叶绿体部分都包含这四个主要成分，但系统 1 制剂相对含有更多的叶绿素 a 684。叶绿素 a 677 和叶绿素 a 684 在系统 1 中具有更大的带宽。在 693 和 704 纳米附近的较长波长处也经常出现波段，但高度远小于上述主要波段。在系统 1 中，长波长波段比系统 2 更常见。在系统 1 中，典型光谱中四个主要波段的半宽度平均为 11.3、10.0、10.3 和 10.8 纳米，而在系统 2 中它们分别为 11.6、9.8、9.4 和 9.6 纳米。发现了一些具有更窄和更宽波段的光谱，但波长相同。
