Moorhead G B, Plaxton W C
Department of Biology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Plant Physiol. 1988 Feb;86(2):348-51. doi: 10.1104/pp.86.2.348.
Numerous studies have demonstrated a rapid increase in the respiration rate during aging of slices of tuber and storage roots. To determine the molecular mechanisms of this phenomenon, the role of enzyme binding to the subcellular particulate fraction has been assessed in carrot (Daucus carota L.) and sugar beet (Beta vulgaris L.). Soluble versus particulate fractions were separated by centrifugation at 16,000g and both fractions assayed for the activities of six glycolytic enzymes. Preparations from sliced and aged tissues showed elevated percentages of five enzymes associated with the particulate fraction as compared with controls. The stimulation of respiration which occurs during aging of underground storage organ slices may result, in part, from an association of enzymes with the particulate fraction of the cell promoting an elevated glycolytic rate.
大量研究表明,块茎和贮藏根切片在老化过程中呼吸速率会迅速增加。为了确定这一现象的分子机制,在胡萝卜(Daucus carota L.)和甜菜(Beta vulgaris L.)中评估了酶与亚细胞颗粒部分结合的作用。通过在16,000g下离心分离可溶性部分和颗粒部分,并对这两个部分进行六种糖酵解酶活性的测定。与对照相比,来自切片和老化组织的制剂显示与颗粒部分相关的五种酶的百分比升高。地下贮藏器官切片老化过程中发生的呼吸刺激可能部分是由于酶与细胞颗粒部分的结合促进了糖酵解速率的提高。