Balhorn R, Jackson V, Granner D, Chalkey R
Biochemistry. 1975 Jun 3;14(11):2504-11. doi: 10.1021/bi00682a033.
The phosphorylating of the lysine-rich histone at various stages in the cell cycle has been studied. In rapidly dividing cell populations the lysine-rich histone is phosphorylated rapidly after synthesis and more slowly once bound to the chromosome. The half-life of hydrolysis of such interphase phosphorylation in 5 hr except during mitosis when the phosphata hydrolysis increases almost three-fold. During mitosis there is extensive phosphorylation at sites different from those phosphorylated during interphase and a smaller measure of sites common to both mitotic and interphase cells. The sites of mitotic phosphorylation are most critically distinguished from those phosphorylated in interphase by the rapidly hydrolysis of M-phase phosphohistone when the cells divide and enter the G1 phase of the cell cycle.
对细胞周期不同阶段富含赖氨酸的组蛋白磷酸化进行了研究。在快速分裂的细胞群体中，富含赖氨酸的组蛋白在合成后迅速磷酸化，一旦与染色体结合则磷酸化较慢。除了在有丝分裂期间磷酸酯水解增加近三倍外，这种间期磷酸化的水解半衰期为5小时。在有丝分裂期间，与间期磷酸化的位点不同，存在广泛的磷酸化，并且有丝分裂细胞和间期细胞共有的位点较少。当细胞分裂并进入细胞周期的G1期时，有丝分裂磷酸化的位点与间期磷酸化的位点最关键的区别在于M期磷酸化组蛋白的快速水解。
