与组蛋白高度乙酰化相关的DNA优先被脱氧核糖核酸酶I消化。
DNA associated with hyperacetylated histone is preferentially digested by DNase I.
作者信息
Sealy L, Chalkley R
出版信息
Nucleic Acids Res. 1978 Jun;5(6):1863-76. doi: 10.1093/nar/5.6.1863.
Abstract
Butyrate-treated cells give rise to massive hyperacetylation of histones and have been used to test the idea that regions of DNA in association with hyperacetylated histones are preferentially solubilized upon digestion with DNase I. Such hyperacetylated histones can be derived from both pre-existing histones or from histone newly synthesized in the presence of butyrate which leads to extreme modification. The DNA in association with both types of hypermodified histone is equally and selectively digested.
摘要
丁酸盐处理的细胞会导致组蛋白大量超乙酰化，并且已被用于检验这样一种观点，即与超乙酰化组蛋白相关的DNA区域在用DNase I消化时优先溶解。这种超乙酰化组蛋白既可以来源于预先存在的组蛋白，也可以来源于在丁酸盐存在下新合成的组蛋白，丁酸盐会导致极端修饰。与这两种超修饰组蛋白相关的DNA被同等且选择性地消化。
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