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与组蛋白高度乙酰化相关的DNA优先被脱氧核糖核酸酶I消化。

DNA associated with hyperacetylated histone is preferentially digested by DNase I.

作者信息

Sealy L, Chalkley R

出版信息

Nucleic Acids Res. 1978 Jun;5(6):1863-76. doi: 10.1093/nar/5.6.1863.

DOI:10.1093/nar/5.6.1863
PMID:673837
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342130/
Abstract

Butyrate-treated cells give rise to massive hyperacetylation of histones and have been used to test the idea that regions of DNA in association with hyperacetylated histones are preferentially solubilized upon digestion with DNase I. Such hyperacetylated histones can be derived from both pre-existing histones or from histone newly synthesized in the presence of butyrate which leads to extreme modification. The DNA in association with both types of hypermodified histone is equally and selectively digested.

摘要

丁酸盐处理的细胞会导致组蛋白大量超乙酰化，并且已被用于检验这样一种观点，即与超乙酰化组蛋白相关的DNA区域在用DNase I消化时优先溶解。这种超乙酰化组蛋白既可以来源于预先存在的组蛋白，也可以来源于在丁酸盐存在下新合成的组蛋白，丁酸盐会导致极端修饰。与这两种超修饰组蛋白相关的DNA被同等且选择性地消化。

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DNA associated with hyperacetylated histone is preferentially digested by DNase I.与组蛋白高度乙酰化相关的DNA优先被脱氧核糖核酸酶I消化。
Nucleic Acids Res. 1978 Jun;5(6):1863-76. doi: 10.1093/nar/5.6.1863.
2
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The identification of distinct populations of acetylated histone.乙酰化组蛋白不同群体的鉴定。
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本文引用的文献

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ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.组蛋白的乙酰化和甲基化及其在RNA合成调控中的可能作用。
Proc Natl Acad Sci U S A. 1964 May;51(5):786-94. doi: 10.1073/pnas.51.5.786.
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Chemical studies of histone acetylation. The distribution of epsilon-N-acetyllysine in calf thymus histones.组蛋白乙酰化的化学研究。小牛胸腺组蛋白中ε-N-乙酰赖氨酸的分布。
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Changing patterns of histone acetylation and RNA synthesis in regeneration of the liver.肝脏再生过程中组蛋白乙酰化和RNA合成模式的变化
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RNA synthesis and histone acetylation during the course of gene activation in lymphocytes.淋巴细胞基因激活过程中的RNA合成与组蛋白乙酰化。
Proc Natl Acad Sci U S A. 1966 Apr;55(4):805-12. doi: 10.1073/pnas.55.4.805.
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Histones: structure and function.组蛋白：结构与功能
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Induction of glycolipid biosynthesis by sodium butyrate in HeLa cells.丁酸钠在HeLa细胞中诱导糖脂生物合成
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Cell cycle-specific changes in histone phosphorylation associated with cell proliferation and chromosome condensation.与细胞增殖和染色体凝聚相关的组蛋白磷酸化的细胞周期特异性变化。
J Cell Biol. 1974 Feb;60(2):356-64. doi: 10.1083/jcb.60.2.356.
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A comparative study of histone acetylation, histone deacetylation, and ribonucleic acid synthesis in avian reticulocytes and erythrocytes.鸟类网织红细胞和红细胞中组蛋白乙酰化、组蛋白去乙酰化及核糖核酸合成的比较研究。
Biochemistry. 1973 Feb 27;12(5):783-91. doi: 10.1021/bi00729a001.
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Regulation of tyrosine hydroxylase activity in cultured mouse neuroblastoma cells: elevation induced by analogs of adenosine 3':5'-cyclic monophosphate.培养的小鼠神经母细胞瘤细胞中酪氨酸羟化酶活性的调节：3':5'-环磷酸腺苷类似物诱导的升高。
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2241-5. doi: 10.1073/pnas.69.8.2241.