Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Plant Physiol. 1988 Nov;88(3):515-7. doi: 10.1104/pp.88.3.515.
A simple, inexpensive technique is described which can be used to pass small electrical currents of a few microamperes through callus cultures under sterile conditions without contaminating the tissue or the medium with electrode products. Application of 1 or 2 microamperes currents of either polarity stimulated growth as well as shoot regeneration in tobacco (Nicotiana tabacum var. virginica) callus cultures, indicating that these effects are due to the electrical fields set up by the exogenous currents, rather than by electrode products.
本文介绍了一种简单、廉价的技术，可在无菌条件下通过愈伤组织培养物传递几微安的小电流，而不会用电极产物污染组织或培养基。施加 1 或 2 微安的任一极性电流均可刺激烟草（Nicotiana tabacum var. virginica）愈伤组织培养物的生长和芽再生，表明这些效应是由外源性电流产生的电场引起的，而不是由电极产物引起的。
