Logemann H, Bergmann L
Botanisches Institut der Universität Köln, Gyrhofstraße 15, D-5000, Köln 41, Federal Republic of Germany.
Planta. 1974 Jan;121(3):283-92. doi: 10.1007/BF00389328.
Light increases the plating efficiency of single cells isolated from green callus cultures of Nicotiana tabacum var. "Samsun" and plated in densities of less than 100 cells ml(-1) by more than 100%. At optimal light intensities plating efficiences between 80 and 90% were achieved with green cells, whereas the growth of isolated cells from chlorophyll free callus cultures was inhibited by light.Plated at low densities the isolated tobacco cells require the addition of kinetin and glutamine to a modified Murashige-Skoog-Medium for growth. The addition of these two substances is not necessary in the case of callus cultures or at higher plating densities (i.e. 500 or more cells ml(-1)). The requirement of kinetin for the growth of isolated cells can be met by the addition of small amounts of conditioned media from suspension cultures to the modified M+S-medium supplemented with glutamine, which demonstrates the production and release of a highly active cytokinine by the cells.
光照可使从烟草品种“萨姆松”绿色愈伤组织培养物中分离出的单细胞的平板接种效率提高100%以上，这些单细胞以低于100个细胞/毫升的密度接种。在最佳光照强度下，绿色细胞的平板接种效率可达80%至90%，而来自无叶绿素愈伤组织培养物的分离细胞的生长则受到光照抑制。以低密度接种时，分离出的烟草细胞需要在改良的Murashige-Skoog培养基中添加激动素和谷氨酰胺才能生长。对于愈伤组织培养或在较高接种密度（即500个或更多细胞/毫升）的情况下，不需要添加这两种物质。通过向添加了谷氨酰胺的改良M+S培养基中添加少量来自悬浮培养物的条件培养基，可以满足分离细胞生长对激动素的需求，这表明细胞产生并释放了一种高活性细胞分裂素。
