Christou P, Barton K A
Agracetus, 8420 University Green, Middleton, Wisconsin 53562.
Plant Physiol. 1989 Feb;89(2):564-8. doi: 10.1104/pp.89.2.564.
A series of structurally related substituted phenethylamines shows extreme toxicity toward wild-type callus tissue cultures of tobacco (Nicotiana tabacum), soybean (Glycine max), corn (Zea mays), and sunflower (Helianthus annuus L.), but tobacco crown gall cultures are resistant to the compounds. The essential components that result in toxicity of the phenethylamines include one aromatic hydroxyl and one primary aliphatic amino group. A series of attenuated crown gall cultures, generated by transformation of tobacco with various modified Agrobacterium strains, has been used to demonstrate that the resistance of crown galls to the phenethylamines is due to the expression in these tissues of isopentenyl transferase, a first step in cytokinin biosynthesis. The toxicity of the compounds to tissue cultures is very rapid, but can be overcome by prior exposure of the calli to exogenous cytokinin. Because of the relationships we have observed between cytokinins and these compounds, we propose that the substituted phenethylamines may represent a class of chemicals that can be used as specific probes to further an understanding of cytokinin metabolism in plant tissues.
一系列结构相关的取代苯乙胺对烟草（Nicotiana tabacum）、大豆（Glycine max）、玉米（Zea mays）和向日葵（Helianthus annuus L.）的野生型愈伤组织培养物表现出极高的毒性，但烟草冠瘿培养物对这些化合物具有抗性。导致苯乙胺毒性的关键成分包括一个芳香羟基和一个伯脂肪族氨基。通过用各种改良的农杆菌菌株转化烟草产生的一系列弱化冠瘿培养物，已被用于证明冠瘿对苯乙胺的抗性是由于这些组织中异戊烯基转移酶的表达，这是细胞分裂素生物合成的第一步。这些化合物对组织培养物的毒性非常迅速，但可以通过愈伤组织预先暴露于外源细胞分裂素来克服。由于我们观察到细胞分裂素与这些化合物之间的关系，我们提出取代苯乙胺可能代表一类化学物质，可作为特定探针用于进一步了解植物组织中的细胞分裂素代谢。
