Teutonico R A, Dudley M W, Orr J D, Lynn D G, Binns A N
Plant Science Institute, Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
Plant Physiol. 1991 Sep;97(1):288-97. doi: 10.1104/pp.97.1.288.
Dehydrodiconiferyl alcohol glucosides (DCGs) are derivatives of the phenylpropanoid pathway that have been isolated from Catharansus roseus L. (Vinca rosea) crown gall tumors. Fractions containing purified DCGs have been shown previously to promote the growth of cytokinin-requiring tissues of tobacco in the absence of exogenous cytokinins. In this study, we utilized synthetic DCG isomers to confirm the cell division-promoting activity of DCG isomers A and B and show that they neither promote shoot meristem initiation on Nicotiana tabacum L., cv Havana 425, leaf explants nor induce betacyanin synthesis in amaranth seedlings. Analysis of cultured tobacco pith tissue demonstrated that DCG accumulation was stimulated by cytokinin treatment and correlated with cytokinin-induced cell division. Thus, the accumulation of metabolites that could replace cytokinin in cell division bioassays is stimulated by cytokinins. These data support the model that DCGs are a component of a cytokinin-mediated regulatory circuit controlling cell division.
脱氢二松柏醇葡萄糖苷（DCGs）是苯丙烷类途径的衍生物，已从长春花的冠瘿瘤中分离出来。先前已表明，含有纯化DCGs的馏分在没有外源细胞分裂素的情况下能促进烟草中需要细胞分裂素的组织的生长。在本研究中，我们利用合成的DCG异构体来确认DCG异构体A和B的促细胞分裂活性，并表明它们既不促进烟草品种哈瓦那425叶片外植体上的茎尖分生组织起始，也不诱导苋菜幼苗中的甜菜色素合成。对培养的烟草髓组织的分析表明，细胞分裂素处理可刺激DCG积累，且与细胞分裂素诱导的细胞分裂相关。因此，细胞分裂素可刺激在细胞分裂生物测定中能够替代细胞分裂素的代谢物的积累。这些数据支持了DCGs是控制细胞分裂的细胞分裂素介导的调节回路的一个组成部分的模型。
