液泡酸水解作为蔗糖分解的一种生理机制。
Vacuolar Acid hydrolysis as a physiological mechanism for sucrose breakdown.
作者信息
Echeverria E, Burns J K
机构信息
University of Florida, IFAS.
出版信息
Plant Physiol. 1989 Jun;90(2):530-3. doi: 10.1104/pp.90.2.530.
Abstract
Sucrose breakdown in mature acidic ;Persian' limes (Citrus aurantifolia [Christm.] Swing.) occurred at a rate of 30.6 picomoles per milliliter per day during 9 weeks storage at 15 degrees C. Neither enzyme of sucrose catabolism (sucrose synthase or acid/alkaline invertase) was present in extracts of mature storage tissue. The average vacuolar pH, estimated by direct measurement of sap from isolated vacuoles and by the methylamine method, was about 2.0 to 2.2. In vitro acid hydrolysis of sucrose at physiological concentrations in a buffered solution (pH 2.2) occurred at identical rates as in matured limes. The results indicate that sucrose breakdown in stored mature acidic limes occurs by acid hydrolysis.
摘要
成熟酸性“波斯”酸橙（Citrus aurantifolia [Christm.] Swing.）在15摄氏度下储存9周期间，蔗糖以每天每毫升30.6皮摩尔的速率分解。成熟储存组织提取物中不存在蔗糖分解代谢的两种酶（蔗糖合酶或酸性/碱性转化酶）。通过直接测量分离液泡中的汁液和甲胺法估算的平均液泡pH约为2.0至2.2。在缓冲溶液（pH 2.2）中生理浓度的蔗糖体外酸水解速率与成熟酸橙中的相同。结果表明，储存的成熟酸性酸橙中的蔗糖分解是通过酸水解发生的。
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