Biotechnology Center, Ohio State University, Columbus, Ohio 43210-1002.
Plant Physiol. 1989 Oct;91(2):690-3. doi: 10.1104/pp.91.2.690.
Previous researchers found that formation and function of nitrogen-fixing nodules on legume roots were severely inhibited by addition of exogenous ethylene. Nodule formation by Rhizobium meliloti on Medicago sativa was stimulated twofold when the ethylene biosynthesis inhibitor aminoethoxyvinylglycine (AVG) was added with the inoculum. Stimulation of nodule formation by AVG showed a similar concentration dependence as inhibition of ethylene biosynthesis, suggesting that the primary action of AVG is the inhibition of ethylene biosynthesis. When AVG was added 2 to 3 days after inoculation, the number of nodules formed was still increased. On a per plant basis, however, the average nitrogen fixation was unchanged by AVG treatment and was independent of nodule number.
先前的研究人员发现,外源乙烯的添加严重抑制了豆科植物根部固氮结节的形成和功能。当将乙烯生物合成抑制剂氨基乙氧基乙烯基甘氨酸(AVG)添加到接种物中时,根瘤菌在紫花苜蓿上形成的根瘤增加了两倍。AVG 对根瘤形成的刺激作用与乙烯生物合成的抑制作用具有相似的浓度依赖性,这表明 AVG 的主要作用是抑制乙烯的生物合成。当 AVG 在接种后 2 至 3 天添加时,形成的根瘤数量仍然增加。然而,在每株植物的基础上,AVG 处理并未改变平均氮固定量,且与根瘤数量无关。