Texas Agricultural Experiment Station, Texas A & M University Research and Extension Center, 17360 Coit Rd., 75252-6599, Dallas, TX, USA.
Plant Cell Rep. 1995 Jan;15(1-2):143-6. doi: 10.1007/BF01690272.
This report describes the regeneration response of excised seedling roots of silktree (Albizzia julibrissin) to added ethylene precursors/generators (1-amino-cyclopropane-1-carboxylic acid [ACC], 2-chloroethylphosphonic acid [CEPA]), biosynthesis inhibitors (aminoethoxyvinylglycine [AVG], an oxime ether derivative [OED={[(ispropylidene)-amino]oxy}-acetic acid-2-(methoxy)-2-oxoethyl ester], CoCl(2) [Co(++)]), and an ethylene action inhibitor (AgNO3 [Ag(+)]). When placed on B5 medium, about 50% of the control explants formed shoot buds within 15 days. Addition of ACC or CEPA (1-10 µM) to the culture medium decreased both the percentage of cultures forming shoots and the number of shoots formed per culture. In contrast, AVG and OED (1-10 µM) increased shoot formation to almost 100% and increased the number of shoots formed per culture. Likewise, both Co(++) and Ag(+) (1-10 µM) increased shoot regeneration, but the number of shoots produced after 30 days was less than with AVG or OED. The inhibitors of ethylene biosynthesis were partially effective in counteracting the inhibitory effect of ACC on shoot formation. These results suggest that modulation of ethylene biosynthesis and/or action can strongly influence the formation of adventitious shoots from excised roots of silktree.
本报告描述了离体丝棉木（Albizzia julibrissin）幼苗根对添加的乙烯前体/发生器（1-氨基环丙烷-1-羧酸[ACC]、2-氯乙基膦酸[CEPA]）、生物合成抑制剂（氨基乙氧基乙烯基甘氨酸[AVG]、肟醚衍生物[OED={[(异丙叉基)氨基]氧基}-乙酸-2-(甲氧基)-2-氧代乙基酯]、CoCl2[Co(++)]）和乙烯作用抑制剂（AgNO3[Ag(+)]）的再生反应。当置于 B5 培养基上时，约 50%的对照外植体在 15 天内形成芽。将 ACC 或 CEPA（1-10 μM）添加到培养基中，降低了形成芽的培养物的百分比和每个培养物形成的芽数。相比之下，AVG 和 OED（1-10 μM）将芽形成增加到几乎 100%，并增加了每个培养物形成的芽数。同样，Co(++)和 Ag(+)（1-10 μM）都增加了芽再生，但 30 天后产生的芽数少于 AVG 或 OED。乙烯生物合成抑制剂在拮抗 ACC 对芽形成的抑制作用方面具有部分效果。这些结果表明，调节乙烯生物合成和/或作用可以强烈影响离体丝棉木根中不定芽的形成。
