Goren R, Bukovac M J, Flore J A
Department of Horticulture, Michigan State University, East Lansing, Michigan 48824.
Plant Physiol. 1974 Feb;53(2):164-6. doi: 10.1104/pp.53.2.164.
Formation of indole-3-acetic acid-aspartate in detached primary leaves of cowpea (Vigna sinensis Endl.) floating on (14)C-indole-3-acetic acid (3 muc; 3.15 mum, phosphate-citrate buffer, pH 4.75), almost doubled when leaves were pretreated with 31.5 mum(12)C-indole-3-acetic acid for 17 hr and then transferred to (14)C-indole-3-acetic acid for 4 hours as compared with leaves preincubated in buffer only. When leaves were preincubated with ethylene (11.0 and 104 mul/l) instead of (12)C-indole-3-acetic acid, no induction of indole-3-acetylaspartic acid formation was observed, and the rate of indole-3-acetylaspartic acid formation decreased as compared with control leaves. Rhizobitoxine (1.87 mum) inhibited indole-3-acetic acid-induced ethylene production but did not prevent the formation of indole-3-acetylaspartic acid. In view of the similarity of these results and those previously obtained with alpha-naphthaleneacetic acid, it is concluded that ethylene has no role in the auxin-induced indole-3-acetylaspartic acid formation in cowpea leaves.
漂浮在含（14）C-吲哚-3-乙酸（3μM；3.15μmol，磷酸盐-柠檬酸盐缓冲液，pH 4.75）中的豇豆（Vigna sinensis Endl.）离体初生叶中吲哚-3-乙酸-天冬氨酸的形成，与仅在缓冲液中预孵育的叶片相比，当叶片先用31.5μM（12）C-吲哚-3-乙酸预处理17小时，然后转移到（14）C-吲哚-3-乙酸中4小时时，其形成量几乎增加了一倍。当叶片用乙烯（11.0和104μl/l）而不是（12）C-吲哚-3-乙酸预孵育时，未观察到吲哚-3-乙酰天冬氨酸形成的诱导，并且与对照叶片相比，吲哚-3-乙酰天冬氨酸的形成速率降低。根瘤毒素（1.87μM）抑制吲哚-3-乙酸诱导的乙烯产生，但不阻止吲哚-3-乙酰天冬氨酸的形成。鉴于这些结果与先前用α-萘乙酸获得的结果相似，得出结论：乙烯在豇豆叶片中生长素诱导的吲哚-3-乙酰天冬氨酸形成中不起作用。
