Department of Biological Sciences, Hunter College, CUNY, New York, New York 10021.
Plant Physiol. 1990 Feb;92(2):358-62. doi: 10.1104/pp.92.2.358.
Microcystis aeruginosa, a unicellular cyanobacterium, contains small phycobilisomes consisting of C-phycocyanin, allophycocyanin, and linker polypeptides. SDS-polyacrylamide gels of the phycobilisomes were examined for fluorescent bands before and after spraying with a solution of ZnSO(4), followed by Coomassie brilliant blue staining for protein. This procedure provides a rapid and sensitive method for detecting small amounts of phycobilin-containing polypeptides and distinguishing them from other tetrapyrrole-containing polypeptides and from ;colorless' ones. Three polypeptide bands, in addition to the alpha and beta phycobiliprotein subunits, have been detected under these conditions. An 85 kilodalton polypeptide was identified as a phycobiliprotein due to its enhanced fluorescence in the presence of ZnSO(4). The other polypeptides do not contain chromophores and are colorless. They are approximately 34.5 and 30 kilodaltons in size.
铜绿微囊藻是一种单细胞蓝藻，含有由藻蓝蛋白、别藻蓝蛋白和连接多肽组成的小藻胆体。藻胆体的 SDS-聚丙烯酰胺凝胶在喷 ZnSO4 溶液前后进行荧光带检查，然后用考马斯亮蓝染色检测蛋白质。该方法提供了一种快速灵敏的方法来检测含藻胆素的多肽，并将其与其他四吡咯含多肽以及“无色”多肽区分开来。在这些条件下，除了 alpha 和 beta 藻胆蛋白亚基外，还检测到了 3 条多肽带。由于在存在 ZnSO4 的情况下其荧光增强，一种 85 千道尔顿的多肽被鉴定为藻胆蛋白。其他多肽不含生色团，呈无色。它们的大小约为 34.5 和 30 千道尔顿。
