Waksman Institute, Rutgers University, Piscataway, New Jersey 08855-0759.
Plant Physiol. 1990 Apr;92(4):1241-3. doi: 10.1104/pp.92.4.1241.
Chromoplasts of Capsicum annuum var Albino contain a carotenoid-protein complex, which migrates as a brilliant orange band in gels under conditions of nondenaturing electrophoresis. In a second, denaturing separation, the complex resolves into a principal protein (ChrA) of 58 kilodaltons and several minor proteins of 20 to 55 kilodaltons, which may be adventitiously associated. Analysis of Western blots of both one- and two-dimensional gels showed that the principal protein component of the carotenoid complex is ChrA, a protein previously shown to be located specifically in chromoplast membranes. The identification of ChrA as a carotenoid-binding protein appears to be the first instance of a nonthylakoid, carotenoid-binding protein in higher plants.
彩椒叶绿体中含有类胡萝卜素-蛋白复合物，在非变性电泳条件下，该复合物在凝胶中迁移形成亮橙色条带。在随后的变性分离中，该复合物解析为分子量为 58 千道尔顿的主要蛋白（ChrA）和分子量为 20 至 55 千道尔顿的几个次要蛋白，这些次要蛋白可能是偶然结合的。对一维和二维凝胶的 Western 印迹分析表明，类胡萝卜素复合物的主要蛋白成分是 ChrA，之前的研究表明该蛋白特异性定位于叶绿体膜上。ChrA 作为类胡萝卜素结合蛋白的鉴定似乎是高等植物中非类囊体、类胡萝卜素结合蛋白的首例。
