Oren-Shamir M, Hadjeb N, Newman L A, Price C A
Waksman Institute, Rutgers University, Piscataway, NJ 08855-0759.
Plant Mol Biol. 1993 Feb;21(3):549-54. doi: 10.1007/BF00028812.
Plant geneticists have determined that the color of ripe fruits of sweet peppers (Capsicum annuum L.) is determined by four genes: y, c1, c2 and cl. We have compared the electrophoretic behavior of chromoplast membrane proteins of seven varieties of C. annuum which differ in these genes. ChrA was detected only in the varieties that had a y+ genotype, and was not affected by variations in the other three genes. The identity of ChrA was verified by probing blots of SDS gels with antiserum to ChrA. The second known chromoplast-specific protein, ChrB, was found to be independent of all four genes. No proteins correlating with c1, c2 or cl were detected in either one- or two-dimensional gels.
植物遗传学家已经确定，甜椒（辣椒属）成熟果实的颜色由四个基因决定：y、c1、c2和cl。我们比较了七个辣椒品种的质体膜蛋白的电泳行为，这些品种在这些基因上存在差异。ChrA仅在具有y+基因型的品种中被检测到，并且不受其他三个基因变异的影响。通过用抗ChrA血清探测SDS凝胶印迹来验证ChrA的一致性。第二种已知的质体特异性蛋白ChrB被发现与所有四个基因无关。在一维或二维凝胶中均未检测到与c1、c2或cl相关的蛋白质。
