Department of Biochemistry, Plant Biotechnology Program, University of Maine, Orono, Maine 04469-0131.
Plant Physiol. 1990 May;93(1):264-70. doi: 10.1104/pp.93.1.264.
Potato (Solanum tuberosum L.) tubers subjected to wounding under hypoxic stress do not synthesize RNA species that are induced in response to wounding in aerobic conditions. Further, wound-response proteins fail to be synthesized when wounded tubers are transferred to hypoxic conditions although messenger RNAs which encode them persist for many hours after transfer. Hypoxic stress also prevents the incorporation of [(3)H]thymidine by wounded tubers that occurs in aerobic conditions. In contrast, hypoxic tubers accumulate and translate transcripts of genes whose products are involved in anaerobic metabolism whether or not they are wounded. Both the hypoxic response and the aerobic wound response preclude the synthesis of proteins encoded by messenger RNAs which accumulated during the tuberization process and which can be translated in vitro. Finally, wounding elicits the degradation of a subset of these tuberization-associated transcripts. These data indicate a complex and precise regulation of gene expression at several levels of macromolecular synthesis.
在缺氧应激下受到创伤的马铃薯（Solanum tuberosum L.）块茎不会合成在有氧条件下响应创伤而诱导的 RNA 种类。此外，当受伤的块茎转移到缺氧条件下时，即使在转移后数小时仍存在编码它们的信使 RNA，但仍无法合成创伤应答蛋白。缺氧应激还会阻止在有氧条件下受伤的块茎中掺入[(3)H]胸苷。相比之下，缺氧块茎会积累并翻译其产物参与无氧代谢的基因的转录本，无论它们是否受伤。缺氧反应和有氧创伤反应都排除了在块茎形成过程中积累并可以在体外翻译的信使 RNA 编码的蛋白质的合成。最后，创伤引发了一部分与块茎形成相关的转录本的降解。这些数据表明在大分子合成的几个水平上对基因表达进行了复杂而精确的调控。
