Department of Biology, Faculty of Science, University of Tokyo, Bunkyo-ku, 113, Japan.
Plant Physiol. 1990 May;93(1):343-6. doi: 10.1104/pp.93.1.343.
Using the patch-clamp technique, we recorded single-channel currents across the excised patch of the plasma membrane of Nitellopsis. Both K(+) and Na(+) can pass this channel, but currents were not carried by Cl(-). Upon the addition of ATP or AMP to the cytoplasmic side, the frequency of channel opening decreased. This is the first report on an ATP-regulated channel in plant cells.
采用膜片钳技术,我们记录了从鱼腥藻质膜切取的膜片上的单通道电流。K(+)和 Na(+)都可以通过该通道,但电流不是由 Cl(-)携带的。当向细胞质一侧加入 ATP 或 AMP 时,通道开放的频率降低。这是植物细胞中 ATP 调节通道的首次报道。