Kisnieriene Vilma, Lapeikaite Indre, Sevriukova Olga, Ruksenas Osvaldas
Department of Neurobiology and Biophysics, Faculty of Natural Science, Vilnius University, M. K. Ciurlionio 21/27, 03101, Vilnius, Lithuania.
J Plant Res. 2016 May;129(3):551-8. doi: 10.1007/s10265-016-0794-3. Epub 2016 Feb 13.
The effect of nickel (Ni) on the generation of plant bioelectrical signals was evaluated in Nitellopsis obtusa, a Characean model organism. Conventional glass-microelectrode technique and K(+)-anaesthesia method in current-clamp and voltage-clamp modes were used for the measurement and analysis of electrical parameters. Ni(2+) treatment rapidly influenced the action potential (AP) parameters namely, excitation threshold, AP peak and duration, membrane potential at various voltages and dynamics of ion currents. We conclude that altered electrical signaling pathway in the test organism constituted the early target for Ni toxicity imposition. The observed Ni interference could be ascribed to disturbed [Ca(2+)]cyt content, impaired Cl(-) and K(+) channels activity resulting in decreased excitability and repolarization rate in generated AP.
在轮藻模型生物钝节拟丽藻（Nitellopsis obtusa）中评估了镍（Ni）对植物生物电信号产生的影响。采用传统玻璃微电极技术以及电流钳和电压钳模式下的钾（K⁺）麻醉方法来测量和分析电参数。镍离子（Ni²⁺）处理迅速影响动作电位（AP）参数，即兴奋阈值、AP峰值和持续时间、不同电压下的膜电位以及离子电流动态。我们得出结论，受试生物体内电信号通路的改变构成了镍毒性施加的早期靶点。观察到的镍干扰可能归因于细胞内钙离子（[Ca²⁺]cyt）含量紊乱、氯离子（Cl⁻）和钾离子（K⁺）通道活性受损，导致所产生动作电位的兴奋性和复极化速率降低。
