Kisnieriene Vilma, Lapeikaite Indre, Pupkis Vilmantas
Department of Neurobiology and Biophysics, Faculty of Natural Science, Vilnius University, Vilnius, Sauletekio av. 7, LT-10257, Lithuania.
Funct Plant Biol. 2018 Jan;45(2):132-142. doi: 10.1071/FP16339.
The Nitellopsis obtusa (N.A.Desvaux) J.Groves cell provides a model system for complex investigation of instantaneous effects of various biologically active compounds (BC) on the generation of plant bioelectrical signals in vivo. Experimental evidence using multiple electrical signals as biomarkers of the effects of BC (acetylcholine, asparagine, glutamate, nicotine, aluminium, nickel and cadmium ions) is provided. The effect of BC on membrane transport systems involved in the cell excitability were tested by current clamp, voltage clamp and patch clamp methods. Membrane potential (MP) alterations and action potential (AP) patterns in response to BC were shown to represent the cell state. High discretisation frequency allows precise, high time resolution analysis of real-time processes measuring changes in excitation threshold, AP amplitude and velocity of repolarisation values after application of BC indicating the effect on ion channels involved in AP generation. Application of voltage clamp revealed that changes in AP peak value were caused not only by increment in averaged maximum amplitude of the Cl- current, but in prolonged Cl- channels' opening time also. The cytoplasmic droplet can serve as a model system in which the effects of BC on single tonoplast ion channel can be studied by patch clamping. Investigation of electrical cell-to-cell communication revealed evidence on the electrical signal transduction through plasmodesmata.
钝节拟丽藻（N.A.Desvaux）J.Groves细胞为体内复杂研究各种生物活性化合物（BC）对植物生物电信号产生的瞬时效应提供了一个模型系统。提供了使用多种电信号作为BC（乙酰胆碱、天冬酰胺、谷氨酸、尼古丁、铝、镍和镉离子）效应生物标志物的实验证据。通过电流钳、电压钳和膜片钳方法测试了BC对参与细胞兴奋性的膜转运系统的影响。结果表明，BC引起的膜电位（MP）变化和动作电位（AP）模式代表了细胞状态。高离散频率允许对实时过程进行精确、高时间分辨率的分析，测量应用BC后兴奋阈值、AP幅度和复极化值速度的变化，表明对参与AP产生的离子通道有影响。电压钳应用表明，AP峰值的变化不仅是由于Cl-电流平均最大幅度的增加，还由于Cl-通道开放时间的延长。细胞质滴可作为一个模型系统，通过膜片钳研究BC对单个液泡膜离子通道的影响。细胞间电通讯的研究揭示了通过胞间连丝进行电信号转导的证据。
