Doehlert D C
Seed Biosynthesis Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Northern Regional Research Center, 1815 North University Street, Peoria, Illinois 61604.
Plant Physiol. 1990 May;93(1):353-5. doi: 10.1104/pp.93.1.353.
A new form of fructokinase has been identified from developing maize (Zea mays L.) kernels that utilizes CTP, UTP, and GTP from four to eight times more effectively than ATP at nonlimiting concentrations. Ten millimolar dithiothreitol was necessary to stabilize activity. A second form of fructokinase was nonspecific for nucleoside triphosphate whereas a third form was fairly specific for ATP.
已从发育中的玉米(Zea mays L.)籽粒中鉴定出一种新型果糖激酶,在非限制浓度下,它利用CTP、UTP和GTP的效率比ATP高4至8倍。10毫摩尔二硫苏糖醇对于稳定活性是必需的。第二种形式的果糖激酶对核苷三磷酸不具有特异性,而第三种形式对ATP相当特异。