Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824-1325.
Plant Physiol. 1990 Nov;94(3):1078-83. doi: 10.1104/pp.94.3.1078.
Changes in gene expression occur during cold acclimation in a wide variety of plant species. Here we show that a number of the polypeptides encoded by cold-regulated (cor) genes of Arabidopsis thaliana L. (Heyn) and wheat share the unusual biochemical property that they remain soluble upon boiling in aqueous solution. Further, cDNA cloning in conjunction with Southern and Northern analyses indicate that wheat has a cor gene that is related to Arabidopsis cor47, a gene encoding a 47 kilodalton ;boiling-stable' COR polypeptide. We suggest it is likely that the boiling-stable COR polypeptides have a fundamental role in plants acclimating to cold temperatures and discuss the possibility that they may act as cryoprotectants.
在许多植物物种的冷驯化过程中,基因表达会发生变化。在这里,我们表明拟南芥和小麦的冷调节(cor)基因编码的许多多肽具有不寻常的生化特性,即在水溶液中煮沸后仍保持可溶性。此外,cDNA 克隆结合 Southern 和 Northern 分析表明,小麦有一个与拟南芥 cor47 相关的 cor 基因,该基因编码一个 47 千道尔顿的“煮沸稳定”COR 多肽。我们认为,煮沸稳定的 COR 多肽很可能在植物适应低温环境中发挥着重要作用,并讨论了它们可能作为抗冻保护剂的可能性。