National Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China.
National Engineering Research Centre for Wheat/Henan Technology Innovation Centre of Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Genes (Basel). 2023 Mar 31;14(4):844. doi: 10.3390/genes14040844.
Wheat production is often impacted by pre-winter freezing damage and cold spells in later spring. To study the influences of cold stress on wheat seedlings, unstressed Jing 841 was sampled once at the seedling stage, followed by 4 °C stress treatment for 30 days and once every 10 days. A total of 12,926 differentially expressed genes (DEGs) were identified from the transcriptome. -means cluster analysis found a group of genes related to the glutamate metabolism pathway, and many genes belonging to the bHLH, MYB, NAC, WRKY, and ERF transcription factor families were highly expressed. Starch and sucrose metabolism, glutathione metabolism, and plant hormone signal transduction pathways were found. Weighted Gene Co-Expression Network Analysis (WGCNA) identified several key genes involved in the development of seedlings under cold stress. The cluster tree diagram showed seven different modules marked with different colors. The blue module had the highest correlation coefficient for the samples treated with cold stress for 30 days, and most genes in this module were rich in glutathione metabolism (ko00480). A total of eight DEGs were validated using quantitative real-time PCR. Overall, this study provides new insights into the physiological metabolic pathways and gene changes in a cold stress transcriptome, and it has a potential significance for improving freezing tolerance in wheat.
小麦生产常受到初冬冻害和春末寒害的影响。为了研究冷胁迫对小麦幼苗的影响，本研究在幼苗期采集了未受胁迫的京 841 小麦，然后进行 4°C 胁迫处理 30 天，每隔 10 天采样一次。共鉴定出 12926 个差异表达基因（DEGs）。-means 聚类分析发现了一组与谷氨酸代谢途径相关的基因，许多属于 bHLH、MYB、NAC、WRKY 和 ERF 转录因子家族的基因高度表达。发现了淀粉和蔗糖代谢、谷胱甘肽代谢和植物激素信号转导途径。加权基因共表达网络分析（WGCNA）鉴定出了几个在冷胁迫下幼苗发育过程中涉及的关键基因。聚类树图显示了 7 个不同颜色标记的不同模块。蓝色模块与冷胁迫处理 30 天的样本相关性最高，该模块中的大多数基因富含谷胱甘肽代谢（ko00480）。使用定量实时 PCR 验证了总共 8 个 DEGs。总的来说，本研究为冷胁迫转录组中生理代谢途径和基因变化提供了新的见解，对提高小麦的抗冻性具有潜在意义。
