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Regulation of Vacuolar pH of Plant Cells: II. A P NMR Study of the Modifications of Vacuolar pH in Isolated Vacuoles Induced by Proton Pumping and Cation/H Exchanges.植物细胞液泡pH的调节：II. 质子泵作用和阳离子/氢离子交换诱导的分离液泡中液泡pH变化的磷-31核磁共振研究
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2
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Plant Physiol. 1987 Feb;83(2):262-4. doi: 10.1104/pp.83.2.262.
3
Anaerobiosis and Release from Dormancy in Apple Embryos: Leaching of (+/-) [C]Abscisic Acid and its Metabolites under Aerobic and Anaerobic Conditions.苹果胚胎的无氧呼吸和休眠解除：在有氧和无氧条件下通过淋洗去除 (+/-) [C]脱落酸及其代谢物。
Plant Physiol. 1983 Aug;72(4):1005-10. doi: 10.1104/pp.72.4.1005.
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ABA Uptake in Source and Sink Tissues of Sugar Beet.甜菜源库组织中 ABA 的摄取。
Plant Physiol. 1983 Jun;72(2):430-3. doi: 10.1104/pp.72.2.430.
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Uptake and Release of Abscisic Acid by Isolated Photoautotrophic Mesophyll Cells, Depending on pH Gradients.依赖于pH梯度的离体光合自养叶肉细胞对脱落酸的吸收与释放
Plant Physiol. 1981 Jul;68(1):202-6. doi: 10.1104/pp.68.1.202.
6
Vacuolar pH Measurement in Higher Plant Cells : I. EVALUATION OF THE METHYLAMINE METHOD.高等植物细胞液泡pH值的测量：I. 甲胺法的评估
Plant Physiol. 1981 May;67(5):953-7. doi: 10.1104/pp.67.5.953.
7
Uptake and subcellular compartmentation of gibberellin a(1) applied to leaves of barley and cowpea.赤霉素A(1)施用于大麦和豇豆叶片后的吸收及亚细胞区室化
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Calculation of intracellular pH from the distribution of 5,5-dimethyl-2,4-oxazolidinedione (DMO); application to skeletal muscle of the dog.根据5,5-二甲基-2,4-恶唑烷二酮（DMO）的分布计算细胞内pH值；应用于犬的骨骼肌
J Clin Invest. 1959 May;38(5):720-9. doi: 10.1172/JCI103852.

悬浮培养的三色苋细胞通过载体介导吸收脱落酸

Carrier-Mediated Uptake of Abscisic Acid by Suspension-Cultured Amaranthus tricolor Cells.

作者信息

Bianco-Colomas J, Barthe P, Orlandini M, Le Page-Degivry M T

机构信息

Laboratoire de Physiologie végétale, Université de Nice-Sophia Antipolis, Parc Valrose, 06034 Nice, Cedex, France.

出版信息

Plant Physiol. 1991 Apr;95(4):990-6. doi: 10.1104/pp.95.4.990.

DOI:10.1104/pp.95.4.990
PMID:16668133
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1077642/
Abstract

Abscisic acid (ABA) uptake by Amaranthus tricolor cell suspensions was found to include both a nonsaturable component and a saturable part with K(m) of 3.74 +/- 0.43 micromolar and an apparent V(max) of 1.5 +/- 0.12 nanomoles per gram per minute. These kinetic parameters as well as the uptake by intact cells at 0 degrees C or by frozen and thawed cells, are consistent with operation of a saturable carrier. This carrier-mediated ABA uptake was partially energized by DeltapH: it increased as the external pH was lowered to pH 4.0; it decreased after the lowering of the DeltapH by the proton ionophore carbonylcyanide-m-chlorophenylhydrazone or after the altering of metabolically maintained pH gradient by metabolic inhibitors (KCN, oligomycin). The carrier is specific for ABA among the plant growth regulators tested, is unaffected by (RS)-trans-ABA and was inhibited by (S)-ABA, (R)-ABA, and also by the ABA analog LAB 173711.

摘要

发现三色苋细胞悬浮液对脱落酸（ABA）的吸收包括一个非饱和成分和一个饱和部分，其米氏常数（K(m)）为3.74±0.43微摩尔，表观最大反应速度（V(max)）为每分钟每克1.5±0.12纳摩尔。这些动力学参数以及0℃下完整细胞或冻融细胞的吸收情况，都与一个饱和载体的运作相符。这种载体介导的ABA吸收部分由ΔpH提供能量：随着外部pH降至4.0，吸收增加；在用质子离子载体羰基氰化物间氯苯腙降低ΔpH后，或在用代谢抑制剂（KCN、寡霉素）改变代谢维持的pH梯度后，吸收减少。在所测试的植物生长调节剂中，该载体对ABA具有特异性，不受（RS）-反式ABA影响，但受（S）-ABA、（R）-ABA以及ABA类似物LAB 173711抑制。