将红细胞分离成“Percoll”密度梯度的临床应用。

Clinical utility of fractionating erythrocytes into "Percoll" density gradients.

作者信息

Mosca A, Paleari R, Modenese A, Rossini S, Parma R, Rocco C, Russo V, Caramenti G, Paderi M L, Galanello R

机构信息

Dipt. Sc. Tecnol. Biomediche, Univ. Milano.

出版信息

Adv Exp Med Biol. 1991;307:227-38. doi: 10.1007/978-1-4684-5985-2_21.

DOI:10.1007/978-1-4684-5985-2_21

PMID:1666816

Abstract

Two rapid methods for fractionating the RBC into five or nine layers of increasing density are reported. These procedures have been used to monitor the decline of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activity during the process of red cell aging in normal subjects and in beta-thal carriers, to study transfused patients with G6PD and pyruvate kinase (PK) deficiency and to test the effects of inositol hexaphosphate (IHP) encapsulation on RBC subpopulations.

摘要

报道了两种将红细胞快速分离成五层或九层密度递增层的方法。这些方法已被用于监测正常受试者和β地中海贫血携带者红细胞衰老过程中葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷酸葡萄糖酸脱氢酶（6PGD）活性的下降，研究患有G6PD和丙酮酸激酶（PK）缺乏症的输血患者，并测试肌醇六磷酸（IHP）包封对红细胞亚群的影响。