Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853.
Plant Physiol. 1991 Jun;96(2):349-54. doi: 10.1104/pp.96.2.349.
Experimental protocols used to study the route(s) of phloem loading in source leaves are evaluated; they include the analysis of plasmodesmatal ultrastructure and distribution, mobile compounds in the free space, loading patterns of exogenous sugars, the site of sugar synthesis, and dye coupling. All these methods have drawbacks. There are indications that some plants, especially those that translocate the raffinose series of sugars, load by a symplastic (through plasmodesmata) pathway, but the evidence is weak.
用于研究源叶韧皮部装载途径的实验方案得到了评估；它们包括质膜结构和分布的分析、游离空间中的可移动化合物、外源糖的装载模式、糖合成的部位以及染料偶联。所有这些方法都有其缺点。有迹象表明，一些植物，特别是那些转运棉子糖系列糖的植物，通过胞质途径（通过胞间连丝）进行装载，但证据很薄弱。
