Department of Plant Biology, Ohio State University, 1735 Neil Avenue, Columbus, Ohio 43210.
Plant Physiol. 1991 Jul;96(3):843-7. doi: 10.1104/pp.96.3.843.
Light from fluorescent lamps can induce formaldehyde production and iron deficiency in plant nutrient culture medium. Formaldehyde is produced from EDTA when it is oxidized by the photochemical reduction of ferric iron and it can accumulate to inhibitory levels. The medium becomes iron deficient because iron becomes unchelated and forms an unavailable precipitate as EDTA is oxidized. The combination of light-induced formaldehyde production and loss of available iron reduces the ability of the culture media to support growth of Arabidopsis thaliana roots. Removing ultraviolet and blue wavelengths with a yellow acrylic filter is a simple and effective means of preventing Fecatalyzed photooxidation of EDTA in plant culture media.
荧光灯发出的光可以在植物营养培养基中诱导甲醛的产生和铁的缺乏。甲醛是由 EDTA 产生的，当它被三价铁的光化学还原氧化时，它可以积累到抑制水平。由于铁失去螯合作用并形成不可用的沉淀，因为 EDTA 被氧化，培养基变得缺铁。光诱导甲醛的产生和可用铁的损失降低了培养基支持拟南芥根生长的能力。用黄色丙烯酸滤光片去除紫外线和蓝光是防止植物培养基中 EDTA 被铁催化光氧化的简单有效方法。
