采用气相色谱选择离子监测-质谱分析和免疫金标记法检测玉米醇溶蛋白结合的吲哚乙酸。
Evidence of zein-bound indoleacetic Acid using gas chromatography-selected ion monitoring-mass spectrometry analysis and immunogold labeling.
机构信息
Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio 45221.
出版信息
Plant Physiol. 1991 Aug;96(4):1070-5. doi: 10.1104/pp.96.4.1070.
Commercial zein was base-hydrolyzed and purified extracts were subjected to gas chromatography-selected ion monitoring-mass spectrometry analysis. Indoleacetic acid (IAA) was shown to be released from this storage protein of corn (Zea mays). Isotope dilution using [(13)C(6)]IAA as an internal standard revealed a conservative ratio of 1 mole IAA to 175 moles zein. Immunoelectron micrographs of isolated protein bodies also showed IAA or an IAA-like molecule associated with zein and deposited within these organelles.
商业玉米醇溶蛋白经碱水解和纯化提取物，采用气相色谱-选择离子监测-质谱分析。结果表明，玉米（Zea mays）这种贮藏蛋白会释放出吲哚乙酸（IAA）。用[(13)C(6)]IAA 作为内标物进行同位素稀释，发现 1 摩尔 IAA 与 175 摩尔玉米醇溶蛋白的保守比例。分离的蛋白体的免疫电子显微镜图像也显示，IAA 或与玉米醇溶蛋白相关的 IAA 样分子，并沉积在这些细胞器内。
Zotero 插件