使用针对羧基连接的吲哚乙酸的抗体进行谷物醇溶蛋白的 Western blot 分析。

Western blot analysis of cereal grain prolamins using an antibody to carboxyl-linked indoleacetic Acid.

机构信息

Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio 45221.

出版信息

Plant Physiol. 1991 Aug;96(4):1076-8. doi: 10.1104/pp.96.4.1076.

DOI:10.1104/pp.96.4.1076

PMID:16668300

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1080895/

Abstract

A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor, and Zea mays. IAA or an IAA-like molecule is associated with the ethanolsoluble protein fraction of the seed. Western blotting of commercial zein, the major storage protein of maize, along with physicochemical evidence reported by Leverone et al. ([1991] Plant Physiol, 96: 1070-1075) indicated that IAA is linked with this prolamin. Results suggest that an IAA-prolamin association may be widespread throughout the Poaceae.

摘要

用针对羧基连接的 IAA 产生的单克隆抗体，对来自燕麦、美洲黍、高粱和玉米的种仁贮藏蛋白进行了 Western blot 分析。IAA 或类似 IAA 的分子与种子的乙醇可溶性蛋白部分相关。对玉米主要贮藏蛋白——商业玉米醇溶蛋白的 Western blot 分析，以及 Leverone 等人（[1991] Plant Physiol，96：1070-1075）报道的理化证据表明，IAA 与这种醇溶蛋白相连。结果表明，IAA-醇溶蛋白的关联可能在整个禾本科中普遍存在。