CIBA-GEIGY Agricultural Biotechnology, P.O. Box 12257, 27709-2257, Research Triangle Park, NC, USA.
Plant Cell Rep. 1993 Sep;12(11):607-11. doi: 10.1007/BF00232808.
Maize (Zea mays L.) tassel primordia were used as a target for particle bombardment, to assess the possibility of introducing foreign DNA into male reproductive structures. Transient expression of the β-glucuronidase gene (GUS) or anthocyanin marker genes (C1 and B-Peru) driven by the CaMV 35S promoter was obtained in tassel primordia 24h after bombardment. Gold particles coated with DNA reached stamen primordia tissues, which eventually form the anthers and pollen. Bombarded tassels were also cultured in vitro and GUS activity was detected in the vascular tissue of mature anthers that developed within 4 weeks. This new approach represents a preliminary step toward pollen mediated transformation.
玉米（Zea mays L.）的雄穗小穗原基被用作粒子轰击的靶标，以评估将外源 DNA 导入雄性生殖结构的可能性。用 CaMV 35S 启动子驱动的β-葡萄糖醛酸酶基因（GUS）或花青素标记基因（C1 和 B-Peru）在轰击后 24 小时的雄穗小穗原基中获得瞬时表达。涂有 DNA 的金颗粒到达雄蕊原基组织，这些组织最终形成花药和花粉。轰击的雄穗也在体外培养，并在 4 周内发育的成熟花药的维管束组织中检测到 GUS 活性。这种新方法代表了花粉介导转化的初步步骤。
