用于载体构建和转化水稻原生质体选择的潮霉素抗性基因盒。
Hygromycin resistance gene cassettes for vector construction and selection of transformed rice protoplasts.
机构信息
Department of Plant Pathology and Crop Physiology, College of Agriculture, Louisiana State University, Baton Rouge, Louisiana 70803-1720.
出版信息
Plant Physiol. 1991 Oct;97(2):832-5. doi: 10.1104/pp.97.2.832.
Abstract
Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique EcoRI, Pstl, and Sacll sites in the coding sequence of a hygromycin B phosphotransferase gene (hph) from Escherichia coli were eliminated. The mutated hph genes were used to form gene cassettes flanked by EcoRI-Sacll-Kpnl-Hindlll sites. Hygromycin resistance of wild-type and mutated hph genes was indistinguishable in E. coli and rice protoplast growth assay.
摘要
潮霉素抗性基因盒被设计用于促进植物转化的载体构建。在大肠杆菌的潮霉素 B 磷酸转移酶基因 (hph) 的编码序列中消除了独特的 EcoRI、Pstl 和 Sacll 位点。突变的 hph 基因用于形成两侧为 EcoRI-Sacll-Kpnl-Hindlll 位点的基因盒。在大肠杆菌和水稻原生质体生长测定中，野生型和突变型 hph 基因的潮霉素抗性没有区别。
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