Department of Horticulture, The Pennsylvania State University, University Park, Pennsylvania 16802.
Plant Physiol. 1992 Jan;98(1):97-100. doi: 10.1104/pp.98.1.97.
Low O(2) conditions were obtained by flowing N(2) through the solution in which the tomato plants (Lycopersicon esculentum Mill cv Heinz 1350) were growing. Time course experiments revealed that low O(2) treatments stimulated 1-aminocyclopropane-1-carboxylate (ACC) synthase production in the roots and leaves. After the initiation of low O(2) conditions, ACC synthase activity and ACC content in the roots increased and reached a peak after 12 and 20 hours, respectively. The conversion of ACC to ethylene in the roots was inhibited by low levels of O(2), and ACC was apparently transported to the leaves where it was converted to ethylene. ACC synthase activity in the leaves was also stimulated by low O(2) treatment to the roots, reaching a peak after 24 hours. ACC synthase levels were enhanced by cobalt chloride and aminooxyacetic acid (AOA), although they inhibited ethylene production. Cobalt chloride enhanced ACC synthase only in combination with low O(2) conditions in the roots. Under aeration, AOA stimulated ACC synthase activity in both the roots and leaves. However, in combination with low O(2) conditions, AOA caused a stimulation in ACC synthase activity in the leaves and no effect in the roots.
通过向番茄植株（Lycopersicon esculentum Mill cv Heinz 1350）生长的溶液中通入氮气来获得低氧条件。时程实验表明，低氧处理刺激了根和叶中 1-氨基环丙烷-1-羧酸（ACC）合酶的产生。在低氧条件开始后，根中的 ACC 合酶活性和 ACC 含量分别在 12 小时和 20 小时后增加并达到峰值。低氧水平抑制了 ACC 在根部向乙烯的转化，并且 ACC 显然被运输到叶片中，在那里它被转化为乙烯。低氧处理也刺激了叶片中的 ACC 合酶活性，在 24 小时后达到峰值。钴氯化物和氨基氧乙酸（AOA）增强了 ACC 合酶的水平，尽管它们抑制了乙烯的产生。钴氯化物仅在根中与低氧条件结合时增强 ACC 合酶。在通气条件下，AOA 刺激了根和叶中 ACC 合酶的活性。然而，与低氧条件结合时，AOA 引起叶片中 ACC 合酶活性的刺激，而对根无影响。
