还原性羧酸循环的一些酶及其特性存在于绿藻莱茵衣藻F-60中。
Some Enzymes and Properties of the Reductive Carboxylic Acid Cycle Are Present in the Green Alga Chlamydomonas reinhardtii F-60.
作者信息
Chen C, Gibbs M
机构信息
Institute for Photobiology of Cells and Organelles, Brandeis University, Waltham, Massachusetts 02254.
出版信息
Plant Physiol. 1992 Feb;98(2):535-9. doi: 10.1104/pp.98.2.535.
Abstract
The reductive carboxylic acid cycle, the autotrophic pathway of CO(2) assimilation in prokaryotes (photosynthetic and nonphotosynthetic autotrophic bacteria), was investigated in Chlamydomonas reinhardtii F-60, an algal mutant lacking a complete photosynthetic carbon reduction pathway (C(3)) due to a deficiency in phosphoribulokinase. Evidence was obtained consistent with the presence of the reductive carboxylic acid cycle in F-60. This conclusion is based on the fact that: (a) acetate approximately doubled CO(2) fixation in whole cells (4 micromoles per milligram chlorophyll per hour) and in chloroplasts (32 nanomoles per milligram chlorophyll per hour); and (b) pyruvate synthase, alpha-ketoglutarate synthase, and ATP-citrate lyase, three indicators of the cycle, were found in cell-free extracts.
摘要
还原型羧酸循环是原核生物（光合和非光合自养细菌）中二氧化碳同化的自养途径，我们在莱茵衣藻F-60中对其进行了研究。莱茵衣藻F-60是一种藻类突变体，由于磷酸核酮糖激酶缺乏，缺少完整的光合碳还原途径（C3途径）。我们获得的证据表明F-60中存在还原型羧酸循环。这一结论基于以下事实：（a）乙酸盐使全细胞（每毫克叶绿素每小时4微摩尔）和叶绿体（每毫克叶绿素每小时32纳摩尔）中的二氧化碳固定量增加了约一倍；（b）在无细胞提取物中发现了该循环的三个指标，即丙酮酸合酶、α-酮戊二酸合酶和ATP-柠檬酸裂解酶。
相似文献
1
Some Enzymes and Properties of the Reductive Carboxylic Acid Cycle Are Present in the Green Alga Chlamydomonas reinhardtii F-60.还原性羧酸循环的一些酶及其特性存在于绿藻莱茵衣藻F-60中。
Plant Physiol. 1992 Feb;98(2):535-9. doi: 10.1104/pp.98.2.535.
2
Functional Importance of Arginine 64 in Chlamydomonas reinhardtii Phosphoribulokinase.莱茵衣藻磷酸核酮糖激酶精氨酸 64 位的功能重要性。
Plant Physiol. 1992 Apr;98(4):1285-9. doi: 10.1104/pp.98.4.1285.
3
A reverse KREBS cycle in photosynthesis: consensus at last.光合作用中的逆向克雷布斯循环：终于达成共识。
Photosynth Res. 1990;24:47-53.
4
Active CO(2) Transport by the Green Alga Chlamydomonas reinhardtii.莱茵衣藻对二氧化碳的主动运输
Plant Physiol. 1989 Apr;89(4):1213-9. doi: 10.1104/pp.89.4.1213.
5
Evidence for Chloroplastic Succinate Dehydrogenase Participating in the Chloroplastic Respiratory and Photosynthetic Electron Transport Chains of Chlamydomonas reinhardtii.叶绿体琥珀酸脱氢酶参与莱茵衣藻叶绿体呼吸和光合电子传递链的证据。
Plant Physiol. 1989 Jul;90(3):1084-7. doi: 10.1104/pp.90.3.1084.
6
Photosynthetic Properties of Chloroplasts from Chlamydomonas reinhardii.莱茵衣藻叶绿体的光合特性
Plant Physiol. 1983 Jun;72(2):488-91. doi: 10.1104/pp.72.2.488.
7
Short-Term Metabolite Changes during Transient Ammonium Assimilation by the N-Limited Green Alga Selenastrum minutum.氮素限制的绿藻微小色球藻在短暂铵同化过程中的短期代谢物变化
Plant Physiol. 1989 Oct;91(2):749-55. doi: 10.1104/pp.91.2.749.
8
Evidence for autotrophic CO2 fixation via the reductive tricarboxylic acid cycle by members of the epsilon subdivision of proteobacteria.变形菌纲ε亚群成员通过还原性三羧酸循环进行自养二氧化碳固定的证据。
J Bacteriol. 2005 May;187(9):3020-7. doi: 10.1128/JB.187.9.3020-3027.2005.
9
Autotrophic CO2 fixation via the reductive tricarboxylic acid cycle in different lineages within the phylum Aquificae: evidence for two ways of citrate cleavage.通过还原三羧酸循环在水栖菌门不同谱系中进行自养二氧化碳固定：柠檬酸裂解两种方式的证据。
Environ Microbiol. 2007 Jan;9(1):81-92. doi: 10.1111/j.1462-2920.2006.01118.x.
10
Anaerobic Formation of d-Lactate and Partial Purification and Characterization of a Pyruvate Reductase from Chlamydomonas reinhardtii.厌氧生成 d-乳酸和小球藻丙酮酸还原酶的部分纯化与表征。
Plant Physiol. 1985 Jun;78(2):277-84. doi: 10.1104/pp.78.2.277.
引用本文的文献
1
Perspective on Daniel I. Arnon's contributions to research, 1960-1994.关于丹尼尔·I·阿农1960年至1994年对研究贡献的展望。
Photosynth Res. 1995 Nov;46(1-2):27-35. doi: 10.1007/BF00020412.
2
Fermentation metabolism and its evolution in algae.藻类的发酵代谢及其进化。
Front Plant Sci. 2013 May 22;4:150. doi: 10.3389/fpls.2013.00150. eCollection 2013.
3
Arsenic tolerance in a Chlamydomonas photosynthetic mutant is due to reduced arsenic uptake even in light conditions.在一种小球藻光合突变体中，砷耐受性是由于即使在光照条件下砷摄取减少所致。
Planta. 2012 Nov;236(5):1395-403. doi: 10.1007/s00425-012-1689-8. Epub 2012 Jun 22.
4
The chloroplast proteome: a survey from the Chlamydomonas reinhardtii perspective with a focus on distinctive features.叶绿体蛋白质组：从莱茵衣藻的角度来看的综述，重点介绍其独特特征。
Curr Genet. 2011 Jun;57(3):151-68. doi: 10.1007/s00294-011-0339-1. Epub 2011 Apr 30.
5
The reductive tricarboxylic acid cycle of carbon dioxide assimilation: initial studies and purification of ATP-citrate lyase from the green sulfur bacterium Chlorobium tepidum.二氧化碳同化的还原性三羧酸循环：来自嗜热绿硫细菌绿热栖菌的ATP-柠檬酸裂解酶的初步研究及纯化
J Bacteriol. 1997 Aug;179(15):4859-67. doi: 10.1128/jb.179.15.4859-4867.1997.
6
Photolithoautotrophic growth and control of CO2 fixation in Rhodobacter sphaeroides and Rhodospirillum rubrum in the absence of ribulose bisphosphate carboxylase-oxygenase.在缺乏核酮糖二磷酸羧化酶加氧酶的情况下，球形红杆菌和深红红螺菌的光olitho自养生长及二氧化碳固定的控制
J Bacteriol. 1993 Nov;175(21):7109-14. doi: 10.1128/jb.175.21.7109-7114.1993.
本文引用的文献
1
Localization of the Enzymes Involved in the Photoevolution of H(2) from Acetate in Chlamydomonas reinhardtii.参与醋酸盐的光解产氢反应的酶在莱茵衣藻中的定位。
Plant Physiol. 1989 Jul;90(3):788-91. doi: 10.1104/pp.90.3.788.
2
Hydrogenase-Mediated Activities in Isolated Chloroplasts of Chlamydomonas reinhardii.莱茵衣藻分离叶绿体中氢化酶介导的活性
Plant Physiol. 1986 Feb;80(2):360-3. doi: 10.1104/pp.80.2.360.
3
Fermentative Metabolism of Chlamydomonas reinhardtii: I. Analysis of Fermentative Products from Starch in Dark and Light.莱茵衣藻的发酵代谢：I. 黑暗和光照条件下淀粉发酵产物分析。
Plant Physiol. 1984 May;75(1):212-8. doi: 10.1104/pp.75.1.212.
4
Cellular Fractionation of Chlamydomonas reinhardii with Emphasis on the Isolation of the Chloroplast.用 emphasis 对莱茵衣藻进行细胞分级分离，重点是分离叶绿体。
Plant Physiol. 1983 Jun;72(2):481-7. doi: 10.1104/pp.72.2.481.
5
Characterization of a Photosynthetic Mutant Strain of Chlamydomonas reinhardi Deficient in Phosphoribulokinase Activity.光合突变株的特性红藻纲莱茵衣藻缺乏磷酸核酮糖激酶活性。
Plant Physiol. 1970 Oct;46(4):576-80. doi: 10.1104/pp.46.4.576.
6
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
Plant Physiol. 1949 Jan;24(1):1-15. doi: 10.1104/pp.24.1.1.
7
Carbon dioxide fixation in green sulphur bacteria.绿硫细菌中的二氧化碳固定
Biochem J. 1970 Nov;120(2):399-408. doi: 10.1042/bj1200399.
8
A new ferredoxin-dependent carbon reduction cycle in a photosynthetic bacterium.光合细菌中一种新的依赖铁氧化还原蛋白的碳还原循环。
Proc Natl Acad Sci U S A. 1966 Apr;55(4):928-34. doi: 10.1073/pnas.55.4.928.
9
Role of the reductive carboxylic acid cycle in a photosynthetic bacterium lacking ribulose I,5-diphosphate carboxylase.还原型羧酸循环在缺乏核酮糖-1,5-二磷酸羧化酶的光合细菌中的作用。
Biochim Biophys Acta. 1972;283(1):136-45. doi: 10.1016/0005-2728(72)90105-3.
10
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.
Zotero 插件